1V-93357-3A Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2147

1V-93357-3A Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2147

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1V-93359-2C Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2149

1V-93359-2C Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2149

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1V-93359-6B Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2151

1V-93359-6B Cell Line NTCC®转基因人类胚胎干细胞系。细胞株-BioVector细胞库HES2151

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UOSe015-A临床级干细胞株MasterShef11, MstrShef11, MShef11 cell line -BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Cell Line hPSCreg name UOSe015-A Cite as: UOSe015-A (RRID:CVCL_BW83) Alternative name(s) MasterShef11, MstrShef11, MShef11 Cell line type Human embryonic stem cell (hESC) Similar lines No similar lines found. Last update 22nd January 2022 User feedback No feedback available yet. Login to share your feedback, experiences or results with the research community. Provider Generator University of Sheffield (UOS) Contact: Centre for Stem Cell Biology Owner Centre for Stem Cell Biology Distributors University of Sheffield (UOS) Derivation country United Kingdom External Databases BioSamples SAMEA104627969 Cellosaurus CVCL_BW83 Wikidata Q54904044 General Information Publications Canham MA et al. The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines. Scientific reports. 2015 Nov 26;5:17258. Merkle FT et al. Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations. Nature. 2017 May 11;545(7653):229-233. Halliwell JA et al. Nucleosides Rescue Replication-Mediated Genome Instability of Human Pluripotent Stem Cells. Stem cell reports. 2020 Jun 9;14(6):1009-1017. Projects TempoReg: Molecular and metabolic pathways controlling developmental timing. * Is the cell line readily obtainable for third parties? Yes Research use: allowed Clinical use: allowed Commercial use: allowed Donor Information General Donor Information Sex male Phenotype And Disease Related Information (Donor) Diseases No disease was diagnosed. Family history No Is the medical history available upon request? No Is clinical information available? No Karyotyping (Donor) Has the donor karyotype been analysed? No Other Genotyping (Donor) Is there genome-wide genotyping or functional data available? No External Databases (Donor) BioSamples SAMEA104627970 Ethics Was the embryo established purely for research purposes? No Have both parents consented to the use of the embryo for ESC derivation? Yes Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes Was the consent voluntarily given? Yes Has the donor been informed that participation will not directly influence their personal treatment? Yes Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes Do you (Depositor/Provider) hold the original Donor Consent Form? No If you do not hold the Donor Consent Form, do you know who does? Yes Contact information / weblink https://jessopfertility.org.uk/ Alternatives to consent are available? Yes Alternatives to consent Alternative consent approval number Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised Does consent explicitly allow the derivation of pluripotent stem cells? Yes Does consent expressly prevent the derivation of pluripotent stem cells? No Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No How may genetic information associated with the cell line be accessed? No information Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes Name of accrediting authority involved? National Research Ethics Service - North West London Rec-2 Approval number 05/Q0501/63 HESC Derivation Date of derivation 2012-06-21 Embryo stage Blastula with ICM and Trophoblast Supernumerary embryos from IVF treatment? Yes Separation of research and IVF treatment? Yes PGD Embryo? No Expansion status 4 4 Stephenson 2007 ICM morphology B B Stephenson 2007 Trophectoderm morphology a a Stephenson 2007 ZP removal technique Spontaneous Hatching Cell isolation Mechanical Cell seeding Embryo Derived under xeno-free conditions? Yes Derivation under GMP? Yes Available as clinical grade? Yes Culture Conditions Surface coating Laminin-511 then CellSTART Feeder cells No Passage method Mechanically O2 Concentration 20 % CO2 Concentration 5 % Medium Other medium: Base medium: Nutristem Main protein source: Human Serum Albumin Serum concentration: 5 % NutriStem-protocol.pdf Has Rock inhibitor (Y27632) been used at passage previously with this cell line? No Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? No Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? No Characterisation Analysis Of Undifferentiated Cells Marker Expression Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles SSEA-1 No SSEA-3 Yes SSEA-4 Yes TRA 1-60 Yes TRA 1-81 Yes NANOG Yes POU5F1 (OCT-4) Yes Morphology Morphology pictures Differentiation Potency Endoderm Endoderm Ont Id: UBERON_0000925 In vitro spontaneous differentiation Marker Expressed alpha fetal protein Yes Mesoderm Mesoderm Ont Id: UBERON_0000926 In vitro spontaneous differentiation Marker Expressed Desmin Yes Ectoderm Ectoderm Ont Id: UBERON_0000924 In vitro spontaneous differentiation Marker Expressed Beta Tubulin III Yes Microbiology / Virus Screening Mycoplasma Negative Certificate Of Analysis Is there a certificate of analysis available? Yes Passage: Genotyping Karyotyping (Cell Line) Has the cell line karyotype been analysed? Yes 46, XY MstrShef11_-_Karyotype_Bank_Trace_Diagram_V2.pdf Passage number: P3 Karyotyping method: G-Banding Other Genotyping (Cell Line) Is there genome-wide genotyping or functional data available? Yes

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chHES-86人胚胎干细胞NTCC® Human Embryonic stem cell细胞系-BioVector NTCC细胞保藏中心CVCL_B003

chHES-86人胚胎干细胞NTCC® Human Embryonic stem cell细胞系-BioVector NTCC细胞保藏中心CVCL_B003

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