BioVector® WTC-11 (WTC) 人诱导多能干细胞 / BioVector® WTC-11 Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:BioVector® WTC-11（通常简称为 WTC）是一种具有高度科研价值的人源诱导多能干细胞 (iPSC) 系。该细胞系由加州大学旧金山分校（UCSF）的 Bruce Conklin 实验室建立，通过利用 episomal 质粒将人类男性皮肤成纤维细胞重编程而成。WTC-11 被选为 Allen 细胞科学研究所 (Allen Institute for Cell Science) 的核心基准细胞系，用于构建一系列荧光标记的细胞器转基因株系。它以核型稳定、分化效率高以及对 CRISPR/Cas9 基因编辑具有极高的耐受性和效率而著称，是全球细胞生物学、类器官建模和系统生物学研究的“金标准”工具。

BioVector® WTC-11 (often referred to as WTC) is a highly characterized human induced pluripotent stem cell (iPSC) line. Established by the laboratory of Bruce Conklin at UCSF, it was generated by reprogramming human male skin fibroblasts using non-integrating episomal plasmids. WTC-11 was selected as the foundational line for the Allen Institute for Cell Science, where it serves as the parent for a vast collection of fluorescently tagged organelle reporter lines. Renowned for its stable karyotype, high differentiation efficiency, and exceptional amenability to CRISPR/Cas9 gene editing, WTC-11 is a "gold standard" tool in cell biology, organoid modeling, and systems biology.

BioVector® WTC-11 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® WTC-11 人诱导多能干细胞

• 来源组织： 成年男性皮肤成纤维细胞 (Skin Fibroblasts)

• 重编程方式： 非整合型 Episomal 质粒

• 核型： 正常 46, XY

• 主要特点： 基因组高度背景化，特别适合大规模成像和精准基因编辑。

2. 培养条件

• 基础培养基： BioVector® mTeSR Plus 或 Essential 8 (E8)。

• 基质支持： 建议使用 Matrigel 或 hESC-qualified Laminin-511 进行无滋养层培养。

• 培养环境： 37 摄氏度，5% CO2。

• 传代建议： 建议在汇合度达到 70-80% 时进行传代，使用 EDTA (Versene) 进行团块传代。

3. 细胞应用

• 高内涵成像： 作为 Allen 细胞库的亲本株，用于观察亚细胞结构动力学。

• 心肌分化： WTC-11 在分化为功能性心肌细胞（iPSC-CMs）方面具有极高的重现性。

• 疾病建模： 利用 CRISPR 技术引入致病突变，通过同源基因背景研究表型。

4. 注意事项

• 单细胞克隆： 虽然 WTC-11 对单细胞操作耐受良好，但在克隆筛选时必须添加 ROCK 抑制剂 (Y-27632) 以维持成活。

• 分化监测： 定期检测 SSEA-4 和 Tra-1-60 等表面标记物，确保群体的多能性维持在 95% 以上。

English Datasheet

1. General Product Information

• Product Name: BioVector® WTC-11 Human Induced Pluripotent Stem Cell Line

• Source Tissue: Adult male skin fibroblasts

• Reprogramming Method: Non-integrating Episomal plasmids

• Karyotype: Stable 46, XY

• Distinction: The primary line used by the Allen Institute for creating the Integrated Cell collection.

2. Culture Conditions

• Basal Medium: BioVector® mTeSR Plus or Essential 8 (E8) defined media.

• Support Matrix: Feeder-free culture on Matrigel or Laminin-511 (iMatrix-511).

• Incubation: 37°C, 5% CO2.

• Passaging: Clump passaging using EDTA (Versene) is recommended; typically subcultured every 3-4 days at 1:10 to 1:20 ratios.

3. Applications

• Live-Cell Imaging: Ideal for quantitative analysis of cell architecture and organelle interactions.

• Cardiomyocyte Differentiation: Widely recognized as one of the most efficient lines for producing high-purity cardiomyocytes for drug screening.

• Precision Engineering: Optimized for seamless CRISPR-mediated knock-ins and knock-outs.

4. Key Usage Notes

• Genomic Integrity: WTC-11 is valued for its baseline genomic stability; however, regular G-band karyotyping or SNP arrays are advised for long-term cultures.

• Feeding Schedule: For E8 media, daily feeding is mandatory; for mTeSR Plus, weekend-free feeding protocols can be utilized.

注意 / Note: WTC-11 应在生物安全二级 (BSL-2) 条件下操作。该细胞系具有极强的单细胞增殖能力，非常适合用于建立转基因稳定株。WTC-11 should be handled under Biosafety Level 2 (BSL-2) conditions. Due to its superior single-cell survival and proliferative capacity, it is an ideal platform for establishing transgenic stable lines.

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