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载体名称：pCambia1381Xb植物表达载体
基本信息
| 出品公司: | Cambia |
|---|---|
| 载体名称: | pCambia1381Xb, pCambia 1381Xb |
| 质粒类型: | 植物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CAMV35S |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 10647 bp |
| 5 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
| 3 测序引物及序列: | -- |
| 载体标签: | GusA |
| 载体抗性: | 卡那和潮霉素 |
| 筛选标记: | HPTII |
| 备注: | -- |
| 产品目录号: | 1381Xb |
| 稳定性: | 稳定表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
质粒图谱
载体序列
LOCUS pCAMBIA1381Xb 10647 bp ds-DNA circular SYN 01-十二月-2013 DEFINITION Agrobacterium binary vector with hygromycin- and kanamycin-resistance and GUS genes plus the pUC8 MCS. For other reading frames, use pCAMBIA1381Xa or pCAMBIA1381Xc. ACCESSION AF234304 VERSION . KEYWORDS pCAMBIA1381Xb SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI http://www.biovector.com/ COMMENT VNTAUTHORNAME|biovector.com| COMMENT The GenBank record was corrected by inserting a G at position 4541. FEATURES Location/Qualifiers source 1..10647 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 20..1825 /codon_start=1 /gene="uidA?(or gusA)?from E. coli" /product="beta-glucuronidase" /label="GUS" CDS 1832..1849 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 1881..2133 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 2155..2179 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 3479..4108 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 4537..5610 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 6214..6354 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(6540..7128) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7215..8009) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label="KanR" /label="confers resistance to kanamycin" /protein_id=" " misc_feature 8434..8458 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 8536..8710 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(8750..9775) /codon_start=1 /product="hygromycin B phosphotransferase" /label="HygR" /label="confers resistance to hygromycin" promoter complement(9843..10520) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 10609..10644 /label="MCS" /label="multiple cloning site from pUC8" ORIGIN 1 cgctgtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact 61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg 121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt 181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat 241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg 301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga 361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa 421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa 481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat 541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca 601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga 661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca 721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac 781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc 841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg 901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca 961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga 1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt 1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta 1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct 1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga 1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact 1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac 1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag 1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa 1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc 1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg 1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc 1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg 1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga 1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg 1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga 1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg 1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc 2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat 2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag 2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa 2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc 2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga 2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag 2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag 2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg 2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg 2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc 2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg 2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag 2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc 2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac 2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct 2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc 3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag 3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca 3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga 3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga 3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg 3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg 3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc 3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat 3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca 3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca 3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat 3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat 3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt 3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct 3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg 3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct 3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg 4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc 4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc 4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc 4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac 4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa 4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc 4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc 4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg 4501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc 4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag 4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc 4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg 4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc 4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga 4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg 4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta 4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt 5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac 5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag 5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac 5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg 5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac 5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc 5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag 5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac 5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg 5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc 5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac 5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg 5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa 5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc 5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc 5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg 6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca 6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 7201 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa 7261 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc 7321 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc 7381 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg 7441 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc 7501 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga 7561 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga 7621 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc 7681 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc 7741 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc 7801 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta 7861 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc 7921 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc 7981 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt 8041 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat 8101 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata 8161 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc 8221 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt 8281 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc 8341 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc 8401 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa 8461 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc 8521 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt 8581 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac 8641 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat 8701 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg 8761 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat 8821 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg 8881 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa 8941 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg 9001 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca 9061 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct 9121 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga 9181 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat 9241 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca 9301 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt 9361 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca 9421 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg 9481 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt 9541 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt 9601 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga 9661 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga 9721 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc 9781 attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga 9841 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag 9901 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 9961 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 10021 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 10081 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 10141 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 10201 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 10261 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca 10321 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa 10381 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg 10441 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta 10501 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct 10561 tggagtagac gagagtgtcg tgctccacca tgttgggccc ggcgcgccga attcccgggg 10621 atccgtcgac ctgcagccaa gcttcag
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我们拥有目前最大的cDNA克隆库、覆盖30,000个人全长基因的TrueClone cDNA克隆和超过25,000个TrueORF cDNA克隆。利用TrueORF cDNA克隆，我们开发了大量的全长人重组蛋白产品（哺乳动物细胞表达），可以进行蛋白功能的研究。另外，Biovector,Inc提供独特的基因表达产品，如TissueScan cancer qPCR array用于生物标记物的发现及鉴定。
33,000 种人TrueClones 全长人cDNA克隆（无标签）
5,000种 小鼠 TrueClones 全长小鼠cDNA克隆（无标签）
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