出品公司: | |
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载体名称: | pNZ8048 |
质粒类型: | 乳酸菌表达载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3349 |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氯霉素 |
筛选标记: | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳定 |
组成型: | 诱导型 |
病毒/非病毒: | 非病毒 |
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地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNZ8048 3349 bp DNA circular 11-JAN-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers misc_feature 788..997 /vntifkey="21" /label=RepC misc_feature 1266..1964 /vntifkey="21" /label=RepA misc_feature 6..189 /vntifkey="21" /label=Nis\A\Promoter\(PnisA) terminator 394..446 /vntifkey="43" /label=Terminator BASE COUNT 775 a 286 c 430 g 646 t 1212 others ORIGIN 1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa 61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg 121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa 181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga 241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa 301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg 361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag 421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat 481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg 541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg 601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt 661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa 721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg 781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg 841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg 901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt 961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt 1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga 1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg 1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag 1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt 1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact 1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc 1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac 1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg 1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc 1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg 1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt 1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac 1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc 1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa 1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa 1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga 1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga 2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat 2101 tattgaataa ataaaagccc ccctgacgaa agtcgacnnn nnnnnnnnnn nnnnnnnnnn 2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnn
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