pAN8-1质粒载体图谱序列抗性说明书价格Biovector公司

LOCUS       pAN8-1	6089 bp 	DNA	SYN
DEFINITION  pAN8-1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="pAN8-1"
                     /mol_type="other DNA"
     CDS             complement(87..659)
                     /label="ORF frame 1"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     gene            2302..2673
                     /label="bleo"
                     /gene="bleo"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     misc_feature    2302..2676
                     /label="sh_ble"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     promoter        complement(3464..3480)
                     /label="M13_forward20_primer"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     misc_feature    complement(3473..3495)
                     /label="M13_pUC_fwd_primer"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     misc_feature    3473..3616
                     /label="lacZ_a"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     misc_feature    3808..3830
                     /label="pGEX_3_primer"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     promoter        3989..4017
                     /label="AmpR_promoter"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     gene            4059..4919
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLQARVSAWKANPNEAMLPPIPHHLIYNTESKNILLTPLRPPTS
                     IRSLLLLDTTPPVLLGVLEGGLVGLGLALAEKAVGSLKGTLEVTSRWLPEDVDLNQIR
                     KRLQRMIDWIRRALVYLKYRCMTAIMPTPMNWLLMSWRNWPLSYSMLVVVTGRGSSPL
                     PSGAGSTYFSVERSAEITSSQPTANRISRQ*"
     CDS             4059..4919
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5074..5693
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6002..6031
                     /label="lac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6045..6067
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6066..6084
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
   61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
  121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
  181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
  241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
  301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
  361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
  421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
  481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
  541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
  601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
  661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
  721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
  781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
  841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
  901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
  961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
 1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
 1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
 1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
 1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
 1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
 1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
 1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
 1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
 1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
 1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
 1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
 1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
 1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
 1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
 1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
 1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
 1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
 2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
 2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
 2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
 2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
 2281 cccgcttgag cagacatcac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
 2341 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
 2401 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
 2461 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
 2521 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
 2581 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
 2641 aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
 2701 ccgacggcgg cccacgggtc ccaggagctt gagatccact taacgttact gaaatcatca
 2761 aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
 2821 caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
 2881 tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt
 2941 ccagatacag ctcatctgca atgcattaat gcattgactg caacctagta acgccttnca
 3001 ggctccggcg aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc
 3061 aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg
 3121 cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct
 3181 tgactatgaa aattccgtca ccagcncctg ggttcgcaaa gataattgca tgtttcttcc
 3241 ttgaactctc aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcanttc
 3301 ctactaagat ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac
 3361 ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
 3421 aggtacactt gtttagaggt aatccttctt tctagagctt ggcactggcc gtcgttttac
 3481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
 3541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
 3601 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
 3661 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
 3721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
 3781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
 3841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
 3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
 3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
 4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
 4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
 4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
 4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
 4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
 4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
 4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
 4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
 4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
 4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
 4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
 4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
 4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
 4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
 4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
 4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
 4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
 5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
 5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
 5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
 5221 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
 5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
 5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
 5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
 5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
 5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
 5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
 5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
 5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
 5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
 5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
 5881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
 5941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
 6001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
 6061 acacaggaaa cagctatgac catgattac
//