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pSPT19载体图谱:载体序列:LOCUS pSPT19 3107 bp DNA SYN 24-Jun-2014
DEFINITION pSPT19
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3107
/organism="pSPT19"
/mol_type="other DNA"
promoter complement(74..92)
/label="T7_promoter"
misc_feature complement(143..165)
/label="pGEX_3_primer"
rep_origin complement(564..1183)
/label="pBR322_origin"
CDS complement(1338..2198)
/label="Ampicillin"
/gene="Ampicillin"
/note="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
gene complement(1338..2198)
/label="Ampicillin"
/gene="Ampicillin"
promoter complement(2240..2268)
/label="AmpR_promoter"
misc_feature complement(2427..2449)
/label="pGEX_3_primer"
misc_feature 2782..2801
/label="pBRrevBam_primer"
misc_feature 3091..1
/label="Sp6_primer"
ORIGIN
1 gaatacacgg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
61 aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagctg ggcctcgcgc
121 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
181 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
241 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat atactggctt
301 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
361 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
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DEFINITION pSPT19
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3107
/organism="pSPT19"
/mol_type="other DNA"
promoter complement(74..92)
/label="T7_promoter"
misc_feature complement(143..165)
/label="pGEX_3_primer"
rep_origin complement(564..1183)
/label="pBR322_origin"
CDS complement(1338..2198)
/label="Ampicillin"
/gene="Ampicillin"
/note="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
gene complement(1338..2198)
/label="Ampicillin"
/gene="Ampicillin"
promoter complement(2240..2268)
/label="AmpR_promoter"
misc_feature complement(2427..2449)
/label="pGEX_3_primer"
misc_feature 2782..2801
/label="pBRrevBam_primer"
misc_feature 3091..1
/label="Sp6_primer"
ORIGIN
1 gaatacacgg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
61 aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagctg ggcctcgcgc
121 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
181 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
241 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat atactggctt
301 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
361 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
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