pET-17b图谱序列抗性价格Biovector质粒库

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pET17b载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences(Novagen)

pET17b, pet 17b

大肠杆菌蛋白表达

高

多克隆位点，限制性内切酶

3306bp

5'-TAATACGACTCACTATAGGG-3'

N-T7

Ampicillin

Hosts: E.coli. Related vectors: pBR322.

69663-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pET17b载体质粒图谱和多克隆位点信息

pet17b载体图谱

Feature Name
Start
End

T7_terminator	131	3
T7_Terminal_primer	71	89
T7_leader	269	237
Xpress_fwd_primer	270	252
T7_transl_en_RBS	294	278
T7_promoter	349	331
ROP	858	1049
pGEX_3_primer	1065	1043
Ampicillin	3098	2238
AmpR_promoter	3168	3140

ORF
Start
End

ORF frame 2

3098

2238

Enzyme Name
Cut

XhoI	141
NotI	147
EcoRV	166
EcoRI	174
SpeI	199
BamHI	205
SacI	215
KpnI	221
HindIII	223
NheI	261
NdeI	268
XbaI	306
BglII	364
MscI	389
PvuII	1009
AatII	3233

pET17b载体简介

The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pET17b载体序列

LOCUS       pET-17b   3306 bp    DNA   circular SYN
DEFINITION pET-17b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="pET-17b"
                     /mol_type="other DNA"
     terminator      complement(3..131)
                     /label="T7_terminator"
     misc_feature    71..89
                     /label="T7_Terminal_primer"
     misc_feature    complement(237..269)
                     /label="T7_leader"
     misc_feature    complement(252..270)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(278..294)
                     /label="T7_transl_en_RBS"
     promoter        complement(331..349)
                     /label="T7_promoter"
     misc_feature    858..1049
                     /label="ROP"
     misc_feature    complement(1043..1065)
                     /label="pGEX_3_primer"
     gene            complement(2238..3098)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2238..3098)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(3140..3168)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL