KOPN-8 人前B淋巴瘤细胞

价　　格：¥79865
货　　号：KOPN-8 人前B淋巴瘤细胞
产　　地：北京

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KOPN-8 人前B淋巴瘤细胞

Cell line:

KOPN-8

Cat no.:

BioVector-ACC 552

Species:

human (Homo sapiens)

Cell type:

B cell precursor leukemia

Origin:

established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells were described in the literature to carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Biosafety level:

Cell Culture Data:

Morphology:

single round rather small cells growing in suspension;

image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 1.0 x 10⁶ cells/ml in a 12-well-plate with 20% FBS; maintain at ca. 0.5-1.5 x 10⁶ cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 48 hours

Harvest:

cell harvest of ca. 2.0 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 -, HLA-DR +, cyIgG -, cyIgM +; image

Fingerprint:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypodiploid karyotype with 4% polyploidy - 45(42-45)<2n>XX, -14, t(8;13)(q24;q21.2), t(11;19)(q23;p13), der(13)t(13;14)(p11;q11) - carries t(11;19) effecting KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion - matches published karyotype

Molec. Genetics:

expression of fusion gene KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -