A20 小鼠B细胞淋巴瘤细胞     

A20 [A-20] Cell Line 小鼠B细胞淋巴瘤细胞    

Cat No.: BioVector-931722

Product category

Animal cells

Organism

Mus musculus, mouse

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Cell type

B lymphocyte

Morphology

lymphoblast

Disease

Sarcoma; Reticulum Cell

Applications

3D cell culture

Immunology

Specific applications

This cell line is a suitable transfection host.

Characteristics

Growth properties

Suspension

Derivation

The A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.

Strain

BALB/cAnN

Karyotype

diploid; modal number = 37; range = 33 to 38

Tumorigenic

Yes;

Yes

Antigen expression

I-A+

Genes expressed

immunoglobulin (surface, sIg+) I-A+

Expression markers

Fc

Isotype

IgG

Comments

The cells express little surface immunoglobulin when grown in Click's medium; however, they express large amounts when grown in RPMI 1640 medium.

The cells can present both alloantigens and protein antigens.

Tested and found negative for ectromelia virus (mousepox).

DMEM-H +10%FBS+ 100 U/ml penicillin G sodium (option); 100 µg/ml streptomycin sulfate (option);

Complete growth medium, 95%; DMSO, 5%

Handling information

Unpacking and storage instructions

1.Check all containers for leakage or breakage.

2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

The base medium for this cell line isRPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

0.05 mM 2-mercaptoethanol

Temperature

37°C

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

3.Transfer the vial contents to a centrifuge tube containing  9.0 ml complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.

4.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into new culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

5.Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

Subculturing procedure

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.

Population doubling time: Approximately 18 hrs

Medium Renewal: Every 2 to 3 days

Reagents for cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO