pSilencer™ 3.1-H1 neo Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 3.1-H1 neo Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'


pSilencer™ 3.1-H1 neo Restriction Sites (Vector Size: 4285 bp)

Enzymes with single Restriction Sites

SiteEnzyme
4220AatII
915AflIII
1808AhdI
1331AlwNI
3772AvaI
3793AvrII
495BamHI
3226BanII
2313BcgI
2347BcgI`
3742BclI
3283BsaAI
3728BsaBI
790BsaXI
760BsaXI`
3803BseRI
45BsmBI
2667EagI
4274EcoO109I
396EcoRI
556HindIII
3505MscI
184NdeI
2903NruI
915PciI
3538PstI
3068RsrII
2288ScaI
4025SexAI
3774SmaI
3798StuI
3468Tth111I
3772XmaI
2407XmnI
4218ZraI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AccI
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI`
BbsI
BbvCI
BglII
BlpI
BmgBI
BmtI
Bpu10I
BsgI
BsiWI
BspEI
BsrGI
BstBI
BstEII
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
DraIII
EcoICRI
EcoNI
EcoRV
FseI
FspAI
HincII
HpaI
KpnI
MfeI
MluI
NheI
NotI
PacI
PflMI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI`
SacI
SacII
SalI
SanDI
SbfI
SfiI
SgfI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI