pET43.1a(+) plasmid vector原核表达质粒载体

pET43.1a大肠杆菌表达载体NusA融合蛋白表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pET43.1a大肠杆菌表达载体NusA融合蛋白表达质粒，HSV标签S标签，EK蛋白酶切割位点，Amp抗性。

The pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Unique sites are shown on the circle map. Notethat the sequence is numbered by the pBR322 convention, so the T7 expression region is reversedon the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will producevirions containing single stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the ColiDOWN primer. Vectorencoded sequence can be completely removed when cloning into the PshA I or Sma I sites (asshown below) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively

pET-43.1a(+) sequence landmarksT7 promoter 2390-2406T7 transcription start 2390Nus•Tag™ coding sequence 834–2318His•Tag® coding sequence 801–818S•Tag™ coding sequence 747–791Multiple cloning sites(Sma I-Xho I) 721–686HSV•Tag® coding sequence 537–572His•Tag® coding sequence 513–530T7 terminator 27–73lacI coding sequence 2797–3879pBR322 origin 5073bla (Ap) coding sequence 5833–6693f1 origin 6815–7262

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