pcDNA3.1-EGFP质粒载体图谱序列抗性说明书价格报价

LOCUS pcDNA3.1(+)-EGFP 6119 bp DNA circular SYN 21-JUN-2012
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
CDS complement(5123..5983)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1719..1943
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1713..1730)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5984..6082)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 1989..2417
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
CDS 2827..3621
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(4308..4978)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 2422..2791
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3795..3925
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
misc_feature 948..1667
/vntifkey="21"
/label=EGFP
misc_feature 942..947
/vntifkey="21"
/label=Kozak\Sequence
BASE COUNT 1417 a 1649 c 1580 g 1473 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccaccggt cgccaccatg gtgagcaagg
961 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
1021 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
1081 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
1141 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
1201 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
1261 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
1321 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
1381 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
1441 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
1501 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
1561 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
1621 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgctcga
1681 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
1741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
1801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
1861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
1921 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
1981 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
2041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
2101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
2161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
2221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
2281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
2341 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
2401 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
2461 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2521 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2581 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
2641 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
2701 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
2761 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
2821 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2881 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2941 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
3001 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
3061 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
3121 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
3181 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
3241 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
3301 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
3361 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
3421 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
3481 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
3541 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3601 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3661 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
3721 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
3781 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
3841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
3901 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
3961 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
4021 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
4081 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
4141 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4561 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4801 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
4921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
4981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
5761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
5821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
5881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
5941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//