pCMV-HA质粒载体图谱序列抗性说明书价格报价

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pCMVHA载体基本信息

出品公司:载体名称:质粒类型:高拷贝/低拷贝:启动子:克隆方法:载体大小:5' 测序引物及序列:3' 测序引物及序列:载体标签:载体抗性:筛选标记:备注:产品目录号:稳定性:组成型:病毒/非病毒:

Clontech

pCMV-HA

哺乳细胞表达系统载体

高拷贝

CMV

多克隆位点，限制性内切酶

3782bp

pCMV-F: 5'd[GATCCGGTACTAGAGGAACTGAAAAAC]3'

HA (N term)

Ampicillin

用于在哺乳细胞中表达N端带有HA标签的蛋白

631604

瞬表达

组成型

非病毒

pCMVHA载体质粒图谱和多克隆位点信息

pCMV-HA载体图谱

pCMVHA载体简介

pCMVHA载体序列

LOCUS pCMV-HA 3782 bp DNA SYN
DEFINITION pCMV-HA
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3782
/organism="pCMV-HA"
/mol_type="other DNA"
misc_feature 31..318
/label="CAG_enhancer"
promoter 36..528
/label="CMV_immearly_promoter"
misc_feature 485..505
/label="CMV_fwd_primer"
promoter 486..555
/label="CMV_promoter"
promoter 498..617
/label="CMV2_promoter"
misc_feature 529..548
/label="pCEP_fwd_primer"
misc_feature 531..555
/label="LNCX_primer"
misc_feature 631..657
/label="pCMV_primer"
misc_feature 672..768
/label="modSV40_late_16s_int"
misc_feature 672..702
/label="SV40_late_19s_int"
terminator complement(931..1050)
/label="SV40_PA_terminator"
misc_feature complement(945..964)
/label="EBV_rev_primer"
promoter complement(1162..1180)
/label="M13_reverse_primer"
misc_feature complement(1179..1201)
/label="M13_pUC_rev_primer"
promoter complement(1215..1244)
/label="lac_promoter"
gene complement(2327..3187)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2327..3187)
/label="ORF frame 2"
promoter complement(3229..3257)
/label="AmpR_promoter"
misc_feature complement(3416..3438)
/label="pGEX_3_primer"
misc_feature complement(3637..3780)
/label="lacZ_a"
misc_feature 3751..3773
/label="M13_pUC_fwd_primer"
promoter 3766..3782
/label="M13_forward20_primer"
ORIGIN
1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGTCGAC CGAGATCTCT
901 CGAGGTACCG CGGCCGCGGG GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
961 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT
1021 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA
1081 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTCGGA TCCTCTAGAG TCGATCTGCA
1141 GGCATGCTAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
1201 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
1261 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
1321 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
1381 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
1441 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
1501 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
1561 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
1621 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
1681 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
1741 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
1801 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
1861 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
1921 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
1981 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
2041 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
2101 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
2161 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
2221 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
2281 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
2341 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
2401 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
2461 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
2521 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
2581 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
2641 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
2701 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
2761 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
2821 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
2881 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
2941 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
3001 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
3061 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
3121 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
3181 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
3241 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
3301 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
3361 ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT
3421 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
3481 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
3541 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG
3601 TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG
3661 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
3721 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA
3781 GT
//

pCMVHA载体质粒图谱pdf版和相关资料下载

pCMVHA载体应用举例

质粒载体菌株细胞株基因保藏中心-Biovector Science Lab,Inc.可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。如有需要请联系：

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电邮Biovector@163.com
电话18901268599

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产品分类

原核表达载体

pmCherry-C1
p3xFLAG-KV2.1
pGL3-control
pEGFP-1
pDsRed2-N1
pDsRed2-C1
pEF1/V5-HisA
pEF1/myc-His B
pcDNA4/TO
pcDNA4/V5-His A
pcDNA4/HisMax B
pEF6/myc-His C
pEF4/V5-His A
SuperCos1
pDsRED-Monomer-N1
pmCherry-N1
pDsRed2-1
pAcGFP1-1
pLEGFP-C1
pDsRed-Monomer
pEBFP-N1
pGluc-basic
psiCHECK 2
pRNA-H1.1/Adeno
pRNATin-H1.2/Neo
pRNATin-H1.2/Retro
pRNATin-H1.2/Hygro
pEYFP-N1
pECFP-N1
pECFP-C1
pSecTag2 A
pCMV-Tag 3B
pEGFP-N3
pBudCE4.1
pIRESneo3
pcDNA3.1/Myc-His B
pcDNA3.1/Myc-His C
pcDNA3.1/Myc-His A
pcDNA3.1/His C
pcDNA3.1/His B
pVitro2-neo-mcs
pFLAG-CMV2
pCMV-HA
pCMV-myc
pCAGGS
pSG5
pCMV-Tag2 B
pCMV-Tag 5B
pSFV1
pcDNA3.1(+)/Myc HisB
pUB6/V5 His B
pUB6/V5 His A
pUB6/V5 His C
pRL-TK
pNeoEGFP
c-Flag pcDNA3
pcDNA3.1/Hygro(-)
pcDNA6-Myc/His B
pCMV6-Entry
pCMV6-XL4
p3xFLAG-KV4.2
p3xFLAG-KV1.5
p3xFLAG-CMV-10
pIRES
pIRES-EGFP
pCEP4
pGL3 basic
pcDNA3.1(-)
pEGFP-C3
pGL3-Promoter
pcDNA3.1-EGFP
pcDNA3.1(+)
pGCSi/H1/Neo ShGFP
pDsRED2-Mito
pMIR-Report Luciferase
pIRES2-EGFP
pEGFP-C1
pEGFP-N1
PiggyBac Dual promoter
Super PiggyBac Transposase
pBC1
pDsRED2-Nuc
pDsRED2-Peroxi
pEBFP-C1
pECFP-ER
pECFP-Mito
pEYFP-actin
pEYFP-Golgi
pEYFP-Mem
pEYFP-tub
pGL3-Enhancer
pRL-CMV
pRL-SV40
pOG44
pORF-lacZ
pORF-HSV1tk
pEGFP-Actin
pWHERE
pN3
pEGFP-C2
pEGFP-N2
p3xFlag-CMV-14
pIRES-hrGFP-1a
pcDNA3.1/GS
pcDNA3.1/Zeo(+)
pcDNA3.1/Zeo(-)
pIREShyg3
pIRESpuro3
pGenesil-1
pBK-CMV
pNTAP-B
pVAX1
pIRESneo
pIRESneo2
pSI
pCI
pcDNA3.1/Hygro(+)
pBI-CMV1