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pUB6/V5HisB载体基本信息
Invitrogen |
| pUB6/V5 His B |
| 哺乳细胞表达系统载体 |
| 高拷贝 |
| UbC |
| 多克隆位点，限制性内切酶 |
| 5467bp |
| T7 Fwd：5'd[TAATACGACTCACTATAGGG]3' |
| -- |
| 6X His, V5 |
| Ampicillin |
| Blasticidin |
| 可以严格控制表达水平 |
| V25020 |
| 瞬表达 |
| 组成型 |
| 非病毒 |
pUB6/V5HisB载体质粒图谱和多克隆位点信息
pUB6/V5HisB载体简介
pUB6/V5HisB载体序列
LOCUS pUB6/V5/His B 5467 bp DNA SYN
DEFINITION pUB6/V5/His B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5467
/organism="pUB6/V5/His B"
/mol_type="other DNA"
promoter 13..1227
/label="hUbC_promoter"
misc_feature complement(1422..1439)
/label="BGH_rev_primer"
terminator 1425..1652
/label="bGH_PA_terminator"
rep_origin 1715..2021
/label="f1_origin"
misc_feature complement(2135..2155)
/label="pBABE_3_primer"
misc_feature complement(2141..2357)
/label="SV40_enhancer"
promoter 2153..2422
/label="SV40_promoter"
rep_origin 2321..2398
/label="SV40_origin"
misc_feature 2383..2402
/label="SV40pro_F_primer"
promoter 2516..2583
/label="EM7_promoter"
gene 2584..2982
/label="blast"
/gene="blast"
terminator 3143..3262
/label="SV40_PA_terminator"
misc_feature 3231..3250
/label="EBV_rev_primer"
promoter complement(3306..3324)
/label="M13_reverse_primer"
misc_feature complement(3323..3345)
/label="M13_pUC_rev_primer"
promoter complement(3359..3388)
/label="lac_promoter"
rep_origin complement(3697..4316)
/label="pBR322_origin"
gene complement(4471..5331)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4471..5331)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5373..5401)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GACGGATCGG GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCTCCCGCGG GCGCCCCCCT
61 CCTCACGGCG AGCGCTGCCA CGTCAGACGA AGGGCGCAGG AGCGTCCTGA TCCTTCCGCC
121 CGGACGCTCA GGACAGCGGC CCGCTGCTCA TAAGACTCGG CCTTAGAACC CCAGTATCAG
181 CAGAAGGACA TTTTAGGACG GGACTTGGGT GACTCTAGGG CACTGGTTTT CTTTCCAGAG
241 AGCGGAACAG GCGAGGAAAA GTAGTCCCTT CTCGGCGATT CTGCGGAGGG ATCTCCGTGG
301 GGCGGTGAAC GCCGATGATT ATATAAGGAC GCGCCGGGTG TGGCACAGCT AGTTCCGTCG
361 CAGCCGGGAT TTGGGTCGCG GTTCTTGTTT GTGGATCGCT GTGATCGTCA CTTGGTGAGT
421 AGCGGGCTGC TGGGCTGGCC GGGGCTTTCG TGGCCGCCGG GCCGCTCGGT GGGACGGAAG
481 CGTGTGGAGA GACCGCCAAG GGCTGTAGTC TGGGTCCGCG AGCAAGGTTG CCCTGAACTG
541 GGGGTTGGGG GGAGCGCAGC AAAATGGCGG CTGTTCCCGA GTCTTGAATG GAAGACGCTT
601 GTGAGGCGGG CTGTGAGGTC GTTGAAACAA GGTGGGGGGC ATGGTGGGCG GCAAGAACCC
661 AAGGTCTTGA GGCCTTCGCT AATGCGGGAA AGCTCTTATT CGGGTGAGAT GGGCTGGGGC
721 ACCATCTGGG GACCCTGACG TGAAGTTTGT CACTGACTGG AGAACTCGGT TTGTCGTCTG
781 TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT TGGGAGCGCG
841 CGCCCTCGTC GTGTCGTGAC GTCACCCGTT CTGTTGGCTT ATAATGCAGG GTGGGGCCAC
901 CTGCCGGTAG GTGTGCGGTA GGCTTTTCTC CGTCGCAGGA CGCAGGGTTC GGGCCTAGGG
961 TAGGCTCTCC TGAATCGACA GGCGCCGGAC CTCTGGTGAG GGGAGGGATA AGTGAGGCGT
1021 CAGTTTCTTT GGTCGGTTTT ATGTACCTAT CTTCTTAAGT AGCTGAAGCT CCGGTTTTGA
1081 ACTATGCGCT CGGGGTTGGC GAGTGTGTTT TGTGAAGTTT TTTAGGCACC TTTTGAAATG
1141 TAATCATTTG GGTCAATATG TAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC
1201 TGGCCGTTTT TGGCTTTTTT GTTAGACGAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC
1261 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
1321 GCGGTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGCGTACCGG
1381 TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG
1441 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC
1501 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA
1561 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG
1621 CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG
1681 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT
1741 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT
1801 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC
1861 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA
1921 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC
1981 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT
2041 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT
2101 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA
2161 AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
2221 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
2281 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
2341 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
2401 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
2461 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT
2521 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA
2581 ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA
2641 ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC
2701 GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC
2761 GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC
2821 GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT
2881 CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG
2941 ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT AAGCACTTCG TGGCCGAGGA
3001 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
3061 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
3121 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
3181 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
3241 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
3301 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
3361 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
3421 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
3481 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
3541 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
3601 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
3661 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
3721 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
3781 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
3841 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
3901 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
3961 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
4021 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
4081 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
4141 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
4201 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
4261 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
4321 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
4381 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
4441 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
4501 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
4561 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
4621 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
4681 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
4741 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
4801 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
4861 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
4921 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
4981 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
5041 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
5101 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
5161 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
5221 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
5281 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
5341 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
5401 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
5461 TGACGTC
//
DEFINITION pUB6/V5/His B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5467
/organism="pUB6/V5/His B"
/mol_type="other DNA"
promoter 13..1227
/label="hUbC_promoter"
misc_feature complement(1422..1439)
/label="BGH_rev_primer"
terminator 1425..1652
/label="bGH_PA_terminator"
rep_origin 1715..2021
/label="f1_origin"
misc_feature complement(2135..2155)
/label="pBABE_3_primer"
misc_feature complement(2141..2357)
/label="SV40_enhancer"
promoter 2153..2422
/label="SV40_promoter"
rep_origin 2321..2398
/label="SV40_origin"
misc_feature 2383..2402
/label="SV40pro_F_primer"
promoter 2516..2583
/label="EM7_promoter"
gene 2584..2982
/label="blast"
/gene="blast"
terminator 3143..3262
/label="SV40_PA_terminator"
misc_feature 3231..3250
/label="EBV_rev_primer"
promoter complement(3306..3324)
/label="M13_reverse_primer"
misc_feature complement(3323..3345)
/label="M13_pUC_rev_primer"
promoter complement(3359..3388)
/label="lac_promoter"
rep_origin complement(3697..4316)
/label="pBR322_origin"
gene complement(4471..5331)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4471..5331)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5373..5401)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GACGGATCGG GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCTCCCGCGG GCGCCCCCCT
61 CCTCACGGCG AGCGCTGCCA CGTCAGACGA AGGGCGCAGG AGCGTCCTGA TCCTTCCGCC
121 CGGACGCTCA GGACAGCGGC CCGCTGCTCA TAAGACTCGG CCTTAGAACC CCAGTATCAG
181 CAGAAGGACA TTTTAGGACG GGACTTGGGT GACTCTAGGG CACTGGTTTT CTTTCCAGAG
241 AGCGGAACAG GCGAGGAAAA GTAGTCCCTT CTCGGCGATT CTGCGGAGGG ATCTCCGTGG
301 GGCGGTGAAC GCCGATGATT ATATAAGGAC GCGCCGGGTG TGGCACAGCT AGTTCCGTCG
361 CAGCCGGGAT TTGGGTCGCG GTTCTTGTTT GTGGATCGCT GTGATCGTCA CTTGGTGAGT
421 AGCGGGCTGC TGGGCTGGCC GGGGCTTTCG TGGCCGCCGG GCCGCTCGGT GGGACGGAAG
481 CGTGTGGAGA GACCGCCAAG GGCTGTAGTC TGGGTCCGCG AGCAAGGTTG CCCTGAACTG
541 GGGGTTGGGG GGAGCGCAGC AAAATGGCGG CTGTTCCCGA GTCTTGAATG GAAGACGCTT
601 GTGAGGCGGG CTGTGAGGTC GTTGAAACAA GGTGGGGGGC ATGGTGGGCG GCAAGAACCC
661 AAGGTCTTGA GGCCTTCGCT AATGCGGGAA AGCTCTTATT CGGGTGAGAT GGGCTGGGGC
721 ACCATCTGGG GACCCTGACG TGAAGTTTGT CACTGACTGG AGAACTCGGT TTGTCGTCTG
781 TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT TGGGAGCGCG
841 CGCCCTCGTC GTGTCGTGAC GTCACCCGTT CTGTTGGCTT ATAATGCAGG GTGGGGCCAC
901 CTGCCGGTAG GTGTGCGGTA GGCTTTTCTC CGTCGCAGGA CGCAGGGTTC GGGCCTAGGG
961 TAGGCTCTCC TGAATCGACA GGCGCCGGAC CTCTGGTGAG GGGAGGGATA AGTGAGGCGT
1021 CAGTTTCTTT GGTCGGTTTT ATGTACCTAT CTTCTTAAGT AGCTGAAGCT CCGGTTTTGA
1081 ACTATGCGCT CGGGGTTGGC GAGTGTGTTT TGTGAAGTTT TTTAGGCACC TTTTGAAATG
1141 TAATCATTTG GGTCAATATG TAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC
1201 TGGCCGTTTT TGGCTTTTTT GTTAGACGAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC
1261 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
1321 GCGGTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGCGTACCGG
1381 TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG
1441 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC
1501 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA
1561 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG
1621 CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG
1681 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT
1741 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT
1801 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC
1861 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA
1921 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC
1981 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT
2041 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT
2101 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA
2161 AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
2221 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
2281 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
2341 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
2401 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
2461 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT
2521 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA
2581 ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA
2641 ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC
2701 GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC
2761 GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC
2821 GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT
2881 CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG
2941 ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT AAGCACTTCG TGGCCGAGGA
3001 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
3061 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
3121 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
3181 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
3241 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
3301 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
3361 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
3421 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
3481 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
3541 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
3601 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
3661 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
3721 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
3781 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
3841 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
3901 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
3961 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
4021 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
4081 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
4141 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
4201 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
4261 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
4321 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
4381 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
4441 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
4501 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
4561 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
4621 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
4681 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
4741 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
4801 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
4861 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
4921 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
4981 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
5041 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
5101 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
5161 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
5221 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
5281 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
5341 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
5401 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
5461 TGACGTC
//
pUB6/V5HisB载体质粒图谱pdf版和相关资料下载
pUB6/V5HisB载体应用举例
pEE6.4
pcDNA3.1/His A
pCHO1.0
pSV-Beta-Galactosidase
pSV2-DHFR
pmirGLO
pCI-neo
pEYFP-C1
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