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pCX4pur逆病毒载体质粒图谱序列抗性

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Plasmid: pCX4pur

 

Source/Vendor: Tsuyoshi Akagi, Osaka Bioscience Institute
Alt Name: pCX4-puro
Analyze: Sequence
Plasmid Type: Mammalian Expression, Retroviral
Expression Level: High
Cloning Method: Restriction Enzyme
Size: 6076
5 Sequencing 1 Primer: pBMN-5
5 Sequencing 1 Primer Sequence: gcttggatacacgccgc
3 Sequencing 1 Primer: IRES-R
Bacterial Resistance: Ampicillin
Selectable Marker: Puromycin
Notes:
Akagi,T. et al., Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation, PNAS 100 (23), 13567-13572 (2003).
GenBank: AB086386.1
Stable: Stable
Constitutive: Unspecified
Viral/Non-Viral: Retroviral

Generated Plasmid Map

 
6076 bp 1520 1014 507 6076 5570 5064 4557 4051 3545 3039 2532 2026 XhoI (6076) AmpR_promoter DraI (5665) FspI (5310) ORF frame 1 Ampicillin AscI (3818) 5_LTR2

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