BACKBONE

• Vector backbone
  pBABE-Puro
  (Search Vector Database)
• Backbone manufacturer
  Addgene plasmid #1764
• Backbone size (bp)4517
• Modifications to backbone
  Puro was replaced with EGFP using HindIII and ClaI
• Vector type
  Mammalian Expression, Retroviral

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  30°C
• Growth Strain(s)
  Stbl3
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpBABE_5
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials