BACKBONE

• Vector backbone
  modified pMIG
  (Search Vector Database)
• Backbone size (bp)7166
• Modifications to backbone
  modification of pMIG vector to contain WRE element.
• Vector type
  Mammalian Expression, Retroviral

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Growth instructions
  Use stbl3 cells from Invitrogen to decrease chance of recombination.
• Copy number
  Low Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (3)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None
• Tag / Fusion Protein
  • IRES GFP (C terminal on backbone)

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpLXSN 5
(Common Sequencing Primers)

RESOURCE INFORMATION

• Supplemental documents
  • pMIG-w EcoRI/SalI digest
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials