BACKBONE

• Vector backbone
  pMKO.1
  (Search Vector Database)
• Backbone size (bp)6700
• Vector type
  Mammalian Expression, Retroviral, RNAi
• Selectable markers
  GFP

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpLXSN 5
(Common Sequencing Primers)

RESOURCE INFORMATION

• Addgene Notes
  • Addgene diagnostic digest
  • Addgene diagnostic digest
  • Diagnostic digest 3
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials