pUMVC 逆病毒载体质粒图谱序列抗性

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pUMVC
(BioVector-08449)

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PURPOSE

(Empty Backbone) Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

DEPOSITING LAB

Bob Weinberg

PUBLICATION

Stewart et al RNA 2003 Apr;9(4):493-501.

PRICE

$65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pUMVC
(Search Vector Database)

Vector type
Mammalian Expression, Retroviral

GROWTH IN BACTERIA

Bacterial Resistance(s)
Kanamycin

Growth Temperature
37°C

Growth Strain(s)
DH5alpha

Copy number
Low Copy

SEQUENCE INFORMATION

Depositor Sequences

None.

Addgene Sequences

Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
None

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ sequencing primerCMV Forward
(Common Sequencing Primers)

RESOURCE INFORMATION

Supplemental documents

Weinberg-viral

Terms and Licenses

UBMTA

DEPOSITOR COMMENTS

Packaging plasmid for MuLV constructs, MuLV gag and pol (no envelope). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

See pUMVC3-gag-pol for a map/sequence.
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pUMVC was a gift from Bob Weinberg (Addgene plasmid # 8449)

For your References section:

Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA 2003 Apr;9(4):493-501.10.1261/rna.2192803 PubMed 12649500