BACKBONE

• Vector backbone
  MSCV-IRES-Thy1.1
  (Search Vector Database)
• Backbone manufacturer
  Philippa Marrack Lab
• Backbone size (bp)6063
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Thy1.1

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  ccdB Survival
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteattB1 (not destroyed)
• 3′ cloning siteattB2 (not destroyed)
• 5′ sequencing primerpBABE5
• 3′ sequencing primerIRES-R
(Common Sequencing Primers)

RESOURCE INFORMATION

• Addgene Notes
  • Addgene diagnostic digest
• Terms and Licenses
  • UBMTA