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MSCV-IRES-Thy1.1 DEST
(BioVector-017442)
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PURPOSE(Empty Backbone) Destination vector derived from MSCV-IRES-Thy1.1
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
BACKBONE
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Vector backboneMSCV-IRES-Thy1.1
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Backbone manufacturerPhilippa Marrack Lab
- Backbone size (bp)6063
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Vector typeMammalian Expression, Retroviral
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Selectable markersThy1.1
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ cloning siteattB1 (not destroyed)
- 3′ cloning siteattB2 (not destroyed)
- 5′ sequencing primerpBABE5
- 3′ sequencing primerIRES-R (Common Sequencing Primers)
RESOURCE INFORMATION
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Addgene Notes
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Terms and Licenses
DEPOSITOR COMMENTS
Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442) -
For your References section:
FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. Cell. 2006 Jul 28. 126(2):375-87. 10.1016/j.cell.2006.05.042 PubMed 16873067
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