BACKBONE

• Vector backbone
  pAc5.1/V5-His B
  (Search Vector Database)
• Backbone manufacturer
  Invitrogen
• Backbone size (bp)5400
• Modifications to backbone
  A PCR product containing the Firefly Luciferase gene and an artificial poly-linker sequence was ligated into pAC5.1/V5-His B between the 5 KpnI and 3 XhoI sites. Primers: Forward: GGATCGGGGTACCTATGGAAGACGCCAAAAACATAAAG Reverse: CACTAGACTCGAGCGTTACAATTTGGACTTTCCGCC Because of the stop codon, V5 and His Tags are not expressed. Restriction sites 3 of Luciferase are Xho1-XbaI-ApaI-BtgI-SacII-V5-MluI-AgeI-6xHIS-PmeI-DraI-StuI-SacI.
• Vector type
  Insect Expression, Retroviral, Luciferase
• PromoterActin 5C
• Selectable markers
  Hygromycin, Blasticidin
• Tag / Fusion Protein
  • Luciferase (N terminal on backbone)

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License