- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
MSCV IRES Luciferase
(BioVector-018760)
-
PURPOSE(Empty Backbone)
-
DEPOSITING LAB
-
PUBLICATION
-
PRICE$65 (USD)
BACKBONE
-
Vector backboneMSCV IRES Luciferase
- Backbone size (bp)6300
-
Vector typeMammalian Expression, Retroviral, Luciferase
GROWTH IN BACTERIA
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
SEQUENCE INFORMATION
-
Depositor SequencesNone.
-
Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
-
Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerMSCV (Common Sequencing Primers)
RESOURCE INFORMATION
-
A portion of this plasmid was derived from a plasmid made byWarren Pear
-
Terms and Licenses
DEPOSITOR COMMENTS
To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCV IRES Luciferase was a gift from Scott Lowe (Addgene plasmid # 18760)
您正在向 biovector.net 发送关于产品 MSCV IRES Luciferase 逆病毒载体质粒图谱序列抗性 的询问
- 公告/新闻