BACKBONE

• Vector backbone
  pMSCV
  (Search Vector Database)
• Backbone size (bp)6700
• Vector type
  Mammalian Expression, Retroviral, RNAi
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteAgeI (not destroyed)
• 3′ cloning siteEcoRI (not destroyed)
• 5′ sequencing primerpLXSN 5
• 3′ sequencing primerpBABE-3
(Common Sequencing Primers)

RESOURCE INFORMATION

• Supplemental documents
  • Weinberg-viral
• Terms and Licenses
  • UBMTA