pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性-BioVector NTCC保藏中心提供
- 价 格:¥2930
- 货 号:pMSCV-IRES-GFP II pMIG II
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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pMSCV-IRES-GFP II (pMIG II)
(BioVector-052107)
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- PURPOSE(Empty Backbone) a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
- DEPOSITING LABDario Vignali
- PUBLICATIONHolst et al Nat Protoc. 2006;1(1):406-17.
BACKBONE
- Vector backboneMSCV IRES GFP(Search Vector Database)
- Modifications to backboneexpanded MCS
- Vector typeMammalian Expression, Retroviral
Tag / Fusion Protein
IRES-EGFP (C terminal on backbone)
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
SEQUENCE INFORMATION
- Depositor SequencesPartial (1)
- Addgene SequencesPartial (2)
Full plasmid sequence is available only if provided by the depositing laboratory.
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ sequencing primerpLXSN_5_primer
RESOURCE INFORMATION
- Terms and Licenses
UBMTA
Ancillary Agreement for Plasmids Containing FP Materials
DEPOSITOR COMMENTS
The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.
For your Materials & Methods section:
pMSCV-IRES-GFP II (pMIG II) was a gift from Dario Vignali (Addgene plasmid # 52107)For your References section:
Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263
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