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MSCV-CMV-DsRed-IRES-d24EGFP
(BioVector-041944)-
PURPOSE(Empty Backbone) Retroviral expression of DsRed in mammalian cells
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DEPOSITING LAB
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PRICE$65 (USD)
Add to CartAvailable to Academic and Nonprofits OnlyBACKBONE
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Vector backboneMSCV
- Backbone size (bp)7556
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin ; IRES d24EGFP
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameDsRed
- PromoterTRE
CLONING INFORMATION
- Cloning methodGateway Cloning
- 5′ sequencing primerpLXSN-5
- 3′ sequencing primerMSCV-rev (Common Sequencing Primers)
RESOURCE INFORMATION
DEPOSITOR COMMENTS
24 hour half life EGFP
How to cite this plasmidThese plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-CMV-DsRed-IRES-d24EGFP was a gift from Stephen Elledge (Addgene plasmid # 41944) -
For your References section:
Global protein stability profiling in mammalian cells. Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ. Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489. 10.1126/science.1160489 PubMed 18988847
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