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pBabe HcRed
(BioVector-010677)
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PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PRICE$65 (USD)
BACKBONE
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Vector backbonepBABE HcRed
- Backbone size (bp)5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersHcRed
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerpBABE 5
- 3′ sequencing primerpBABE-3 (Common Sequencing Primers)
RESOURCE INFORMATION
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Terms and Licenses
DEPOSITOR COMMENTS
pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe HcRed was a gift from William Hahn (Addgene plasmid # 10677) -
For your References section:
Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784
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