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pBabe HcRed逆病毒载体质粒图谱序列抗性

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  • 货  号:3558877
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pBabe HcRed 
(BioVector-010677)

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Available to Academic and Nonprofits Only

BACKBONE

  • Vector backbone
    pBABE HcRed
  • Backbone size (bp)5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    HcRed

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

SEQUENCE INFORMATION

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

  • Gene/Insert name
    None

CLONING INFORMATION

RESOURCE INFORMATION

DEPOSITOR COMMENTS

pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe HcRed was a gift from William Hahn (Addgene plasmid # 10677)
  • For your References section:

    Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784

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