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pEVX
(BioVector-017666)
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PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
BACKBONE
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Vector backbonepEVX
- Backbone size (bp)6400
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Vector typeMammalian Expression, Retroviral
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primern/a (Common Sequencing Primers)
RESOURCE INFORMATION
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Terms and Licenses
DEPOSITOR COMMENTS
In the Authors Map, plasmid pEVX contains the
EVX proviral DNA (outlined), flanking cellular DNA (bold black line), and
pBR322 DNA (narrow black line). The LTRs are Moloney murine leukemia virus (MoMLV) long terminal repeats (LTRs).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVX was a gift from Michael Botchan (Addgene plasmid # 17666) -
For your References section:
Transformation mediated by the SV40 T antigens: separation of the overlapping SV40 early genes with a retroviral vector. Kriegler M, Perez CF, Hardy C, Botchan M. Cell. 1984 Sep . 38(2):483-91. 10.1016/0092-8674(84)90503-8 PubMed 6088084
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