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pMSCV-TAP
(BioVector-012570)
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PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
BACKBONE
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Vector backbonepMSCV-TAP
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Backbone manufacturerModified from Clontechs pMSCV
- Backbone size (bp)6800
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
- Tag / Fusion Protein
- TAP (N terminal on backbone)
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerMSCV primer (Common Sequencing Primers)
RESOURCE INFORMATION
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A portion of this plasmid was derived from a plasmid made byTAP was PCRd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
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Terms and Licenses
DEPOSITOR COMMENTS
A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5 -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3 and right primer 5 -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3 . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See authors map for more information (Note that the NotI site is not precisely underlined in the authors map).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-TAP was a gift from David Sabatini (Addgene plasmid # 12570) -
For your References section:
mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001PubMed 16919458
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