BACKBONE

• Vector backbone
  pMSCVpuro
  (Search Vector Database)
• Backbone manufacturer
  Clontech
• Backbone size (bp)6300
• Modifications to backbone
  Inserted Reading Frame Cassette A into the retroviral expression vector pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin and Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  ccdB Survival
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodGateway Cloning
• 5′ sequencing primerpLXSN-5
• 3′ sequencing primerMSCV-rev
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA