MG63 cell line细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

MG63 cell line细胞株

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Organism Homo sapiens, human

Tissue bone

Product Format frozen

Morphology fibroblast

Culture Properties adherent

Biosafety Level 1

Disease osteosarcoma

Age 14 years

Gender male

Ethnicity Caucasian

Applications This cell line is a suitable transfection host.

Karyotype This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Clinical Data 14 years

Caucasian

male

Receptor Expression transforming growth factor beta (TGF-beta) RI, expressed

transforming growth factor beta (TGF-beta) RII, expressed

Genes Expressed interferon

Cellular Products interferon

Comments High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D.

Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.

2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5. Add appropriate aliquots of the cell suspension to new culture vessels.

6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C

STR Profile Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11

D16S539: 11

D5S818: 11,12

D7S820: 10

THO1: 9.3

TPOX: 8,11

vWA: 16,19

References Billiau A, et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813

Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net