SUP-M2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

SUP-M2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 NTCC501493 SUP-M2 Cell Line 1 vial. Storage: Liquid Nitrogen
Cell line:
SUP-M2
Species:
human (Homo sapiens)
Cell type:
anaplastic large cell lymphoma
Origin:
derived from the cerebrospinal fluid of a 5-year-old girl with refractory malignant histiocytosis; described to carry the typical t(2;5)(p23;q35) leading to the NPM1-ALK (NPM-ALK) fusion gene and to express CD30, defining the cells as anaplastic large cell lymphoma (ALCL)
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:3 to 1:5 every second day; seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; maximal density of ca. 1.5 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly and with a reduced viability
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, CD3 -, CD4 +, CD13 -, CD14 -, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +;
image




Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded near-diploid karyotype with 3.5% polyploidy - 44-50<2n>XX/XXX, +1, +7, +8, add(X)(q26), der(X;7)(q10;p10), add(1)(q21), der(1)(1pter->q43::1q43->q21::9q31->qter), t(2;5)(p23;q35), der(6)t(6;6)(q27;p11), add(9)(q31), add(11)(q24)/der(11)t(4;11)(?q34;q24), del(19)(q13.2) - extensive subclonal changes incl. dicentric t(1;1) - carries t(2;5) effecting NPM1-ALK (NPM-ALK) fusion - resembles published karyotype
Molec. Genetics:
expression of fusion gene NPM1-ALK (NPM-ALK) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

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