pnfat-ta-luc信号通路报告质粒， BioVector NTCC质粒载体菌种细胞基因保藏中心

pnfat-ta-luc信号通路报告质粒， BioVector NTCC质粒载体菌种细胞基因保藏中心 pnfat-ta-luc信号通路报告质粒，带有NFAT增强子元件，HSV-TK启动子TATA盒，Luciferase萤火虫荧光素酶报告基因，内源性NFAT转录因子与顺势响应增强元件结合后，转录被激活表达荧光报告基因，Amp抗性。pnfat-ta-luc信号通路报告质粒，Location of features:
• Nuclear factor of activated T-cells (NFAT) enhancer element (2): 27–116
• TA minimal promoter (PTA): 136–142
• Firefly luciferase gene:
Luciferase coding sequences:
start codon (ATG): 222–224; stop codon: 1872–1874
• SV40 late mRNA polyadenylation signal: 2025–2030
mRNA 3' end: 2044
• pUC plasmid replication origin: 2423–3066
• Ampicillin resistance gene:
Promoter: –35 region: 4144–4139; –10 region: 4121–4116
Transcription start point: 4109
Ribosome binding site: 4086–4082
β-lactamase coding sequences:
start codon (ATG): 4074–4072; stop codon: 3216–3214
β-lactamase signal peptide: 4074–4006
β-lactamase mature protein: 4005–3217
• f1 single-strand DNA origin (packages the noncoding strand of luc): 4206–4661
• Transcription blocker (TB): 4792–4945
Synthetic polyadenylation site (4): 4792–4840
Transcription pause site from human α2 globin gene (5): 4854–4945
Propagation in E. coli:
• Suitable host strains: DH5α and other general purpose strains. Single-stranded DNA production requires a host containing an F' episome such as JM109.
• Selectable marker: plasmid confers resistance to ampicillin (50 μg/ml) to E. coli hosts.
• E. coli replication origin: pUC
• Copy number: ~500
• Plasmid incompatibility group: pMB1/Col E1
References:
1. Rao, A., et al. (1997) Ann. Rev. Immunol. 15:707–747.
2. Fiering, S., et al. (1990) Genes Devel. 4:1823–1834.
3. Eggermont, J. & Proudfoot, N. (1993) EMBO J. 12:2539–2548.
4. Levitt, N., et al. (1989) Genes Dev. 3:1019–1025.
5. Enriquez-Harris, P., et al. (1991) EMBO J. 10:1833–1842.《br/>BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net