UCOE Mu-P BioVector NTCC质粒载体菌种细胞基因保藏中心

UCOE Mu-P BioVector NTCC质粒载体菌种细胞基因保藏中心 UCOE® elements are methylation-free
CpG-rich DNA sequences derived from
housekeeping genes. The UCOE® element acts
by impacting chromatin organization, allowing
for increased and stable transgene expression
in mammalian cells.
With UCOE® technology, you can:
• Achieve grams of protein in less than 30 days
• Boost expression levels up to 20-fold higher than possible
with conventional vectors
• Enhance industry-standard platforms, such as CHO cells
and CMV promoters. UCOE® expression technology gives a 9- to 56-fold boost in the
production level of a secreted protein (Erythropoietin). A single
UCOE® element placed upstream of the 5’ end of the promoter and
transgene is sufficient to boost expression twenty-fold or more.
CHO-K1 cells transfected with erythropoietin expression constructs
with or without UCOE® expression technology. Drug-selected pools
were assessed for erythropoietin production.
Rapid production of a monoclonal antibody. 3 x 107 CHO-S
cells were transfected with 90 μg of an antibody expression
plasmid either with or without a UCOE® element. Drug
selection was applied and, once sufficient cell number was
generated, cells were seeded at a cell density that would allow
growth to 10 L. On day 16, a small addition was made to a
portion of the cultures (+opti). A fraction of the cells were
grown and the data shown are an extrapolation of the yield if
10 L of culture had been generated.
Because the majority of cells are stable high-expressors, it is much easier and
faster to find high-yielding clones, as opposed to the “needle-in-a-haystack”
approach using conventional vectors. Vectors encoding heavy and light chains
(separate plasmids) were co-transfected into CHO-S cells followed by drug
selection and screening of 96 randomly selected clones.
Description Catalogue No.
UCOE® Expression Vector, Mouse 3.2 kb, Puro 5.04865.0001
UCOE® Expression Vector, Mouse 3.2 kb, Hygro 5.04866.0001
UCOE® Expression Vector, Human, 4 kb, Puro 5.04867.0001. [Supplier供应厂家]BioVector NTCC Inc. http://www.biovector.net [Supplier来源] http://www.biovector.net