pB1H1 BioVector NTCC质粒载体菌种细胞基因保藏中心

pB1H1 BioVector NTCC质粒载体菌种细胞基因保藏中心 Vector backbone pB1H1
(Search Vector Database)
Backbone size w/o insert (bp) 3598
Vector type Bacterial Expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Chloramphenicol
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name Dorsal RHR in pB1H1
Alt name dorsal
Species D. melanogaster (fly)
Insert Size (bp) 887
GenBank IDCG6667
Entrez Gene dl (a.k.a. Dmel_CG6667, CG6667, DL, Dl, Dmel\CG6667, Dor, Dorsal, GSd447, NFkappaB, REL, anon-EST:GressD7, d, dL, fs(2)k10816, mat(2)dorsal)
Tag / Fusion Protein
flag (N terminal on backbone)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site KpnI (not destroyed)
3′ cloning site AvrII (not destroyed)
5′ sequencing primer GATTCGTCGTGCGGCAACCA
3′ sequencing primer GCGCTTCGTTAATACAGATG. References section:

A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng X, Brodsky MH, Wolfe SA. Nat Biotechnol. 2005 Aug . 23(8):988-94. 10.1038/nbt1120 PubMed 16041365. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net