pH3U3-mcs BioVector NTCC质粒载体菌种细胞基因保藏中心

pH3U3-mcs BioVector NTCC质粒载体菌种细胞基因保藏中心 Vector backbone pH3U3
(Search Vector Database)
Backbone size w/o insert (bp) 5790
Vector type Bacterial Expression
Selectable markers URA3, HIS3
GROWTH IN BACTERIA

Bacterial Resistance(s) Kanamycin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Low Copy
GENE/INSERT

Gene/Insert name Multiple cloning site in His3 Ura3 reporter plasmid
Species S. cerevisiae (budding yeast)
Insert Size (bp) 44
GenBank IDDQ515893
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site NotI (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer CAAATATGTATCCGCTCATGAC. Please note that the cloning information for this plasmid has been corrected as of 1/14/09. The 5' cloning site was changed from MluI to NotI. References section:

A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng X, Brodsky MH, Wolfe SA. Nat Biotechnol. 2005 Aug . 23(8):988-94. 10.1038/nbt1120 PubMed 16041365. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net