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pcDNA3.1/GS质粒图谱序列抗性价格报价Biovector NTCC Inc.现货

  • 价  格:¥1920
  • 货  号:BiovectorpcDNA3.1/GS
  • 产  地:北京
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pcDNA3.1GS载体基本信息

出品公司:Invitrogen
载体名称:pcDNA3.1/GS , pcDNA3.1GS
质粒类型:哺乳动物细胞表达载体
启动子:CMV
表达水平:
克隆方法:多克隆位点,限制性内切酶
载体大小:4020 bp
5' 测序引物:  pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:  pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体标签:C-His,  C-V5-Epitope
载体抗性:Ampicillin 氨苄
筛选标记:Neomycin (G418)
备注:
产品目录号:H-K1000
稳定性:瞬表达 Transient
组成型:组成型 Constitutive
病毒/非病毒:非病毒

pcDNA3.1GS载体质粒图谱和多克隆位点信息

pcDNA3.1/GS载体图谱

pcDNA3.1GS载体简介

GeneStorm Expression-Ready Clones are expressed from the vector pcDN3.1/GS. This vector utilizes the strong immediate-early cytomegalovirus (CMV) promoter for high-level, constitutive expression in mammalian cell lines. Each GeneStorm Clone is fused to a C-terminal peptide encoding the V5 epitope for detection with the Anti-V5-HRP Antibody and a 6His tag for purification on metal-chelating resin (i.e. ProBond). The vector also encodes the Zeocin resistance gene for selection in E. coli and for the  creation of stable mammalian cell lines.

pcDNA3.1GS载体序列

LOCUS       pcDNA3.1/GS             4020 bp    DNA     circular   SYN  2-SEP-2003
SOURCE      
 ORGANISM  
COMMENT     This file is created by Vector NTI
           http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
    source          1..4020
                    /invitrogen="138"
                    /vntifkey="98"
    CDS             82..99
                    /invitrogen="1010000"
                    /vntifkey="4"
                    /label=6xHis
    polyA_site      128..352
                    /invitrogen="1870000"
                    /vntifkey="26"
                    /label=BGH\pA
    primer          complement(122..139)
                    /invitrogen="1910000"
                    /vntifkey="27"
                    /label=BGH\reverse\primer
    primer          3876..3896
                    /invitrogen="1930000"
                    /vntifkey="27"
                    /label=CMV\forward\primer
    promoter        3339..3926
                    /invitrogen="2180000"
                    /vntifkey="29"
                    /label=CMV\promoter
    promoter        1216..1283
                    /invitrogen="2340000"
                    /vntifkey="30"
                    /label=EM7\promoter
    rep_origin      398..826
                    /invitrogen="2500000"
                    /vntifkey="33"
                    /label=f1\origin
    rep_origin      complement(2301..2974)
                    /invitrogen="2540000"
                    /vntifkey="33"
                    /label=pUC\origin
    promoter        831..1201
                    /invitrogen="2290000"
                    /vntifkey="29"
                    /label=SV40\early\promoter
    polyA_signal    1788..1918
                    /invitrogen="1840000"
                    /vntifkey="25"
                    /label=SV40\pA
    primer          3970..3989
                    /invitrogen="2100000"
                    /vntifkey="27"
                    /label=T7\primer
    promoter        3970..3986
                    /invitrogen="2370000"
                    /vntifkey="30"
                    /label=T7\promoter
    protein_bind    4016..4020
                    /invitrogen="2460000"
                    /vntifkey="31"
                    /label=TOPO\binding\site
    protein_bind    complement(1..5)
                    /invitrogen="2460000"
                    /vntifkey="31"
                    /label=TOPO\binding\site
    primer          complement(40..60)
                    /invitrogen="2140000"
                    /vntifkey="27"
                    /label=V5\reverse\primer
    CDS             31..72
                    /invitrogen="1350000"
                    /vntifkey="4"
                    /label=V5\epitope
    CDS             1284..1658
                    /invitrogen="1370000"
                    /vntifkey="4"
                    /label=Zeo(R)
BASE COUNT      905 a      1068 c      1049 g       998 t
ORIGIN
       1 aagggcgagc ttcgaggtca cccattcgaa ggtaagccta tccctaaccc tctcctcggt
      61 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     541 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     841 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    1021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    1081 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
    1141 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
    1201 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    1261 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    1321 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    1381 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    1441 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    1501 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    1561 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    1621 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    1681 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    1741 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    1921 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    1981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    2041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    2101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    2161 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    2221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    2281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    2341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    2401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    2461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    2521 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
    2581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    2641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    2701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    2761 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    2821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    2881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    2941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    3001 cacgttaagg gattttggtc atgagcggat acatatttga atgtatttag aaaaataaac
    3061 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    3121 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
    3181 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    3241 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
    3301 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
    3361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    3541 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    3721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
    3781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
    3841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
    3901 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
    3961 tatcgaaatt aatacgactc actataggga gacccaagct ggctagttaa gctcgccctt


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