出品公司: | Clontech |
---|---|
载体名称: | pQCXIH |
质粒类型: | 哺乳动物细胞双顺反子逆病毒基因表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7780bp |
5' 测序引物及序列: | pQC-F: ACGCCATCCACGCTGTTTTGACCT |
3' 测序引物及序列: | pQC-R: AAGCGGCTTCGGCCAGTAACGTTA |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 潮霉素(Hygromycin) |
备注: | 自失活双顺反子逆病毒表达载体,含有IRES-Hygromycin元件,能够同时表达目的蛋白和潮霉素抗性筛选标记,可以用来构建稳表达细胞系。 |
产品目录号: | 631516 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pQCXIH 7780 bp DNA circular 13-NOV-2009 COMMENT Created by Clontech Laboratories COMMENT Cat. No. 631516 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers LTR 1..728 /label=5'\LTR\(CMV/MSV) misc_signal 758..1567 /label=Extended\packaging\signal LTR 4486..4911 /label=3'\MMLV\LTR\(del) rep_origin 5798..5798 /label=pUC\origin misc_marker complement(6558..7418) /label=Amp(R) rep_origin 5412..5477 /label=SV40\origin misc_marker 2905..3939 /label=Hyg(R) promoter 1601..2132 /label=CMV\IE\Promoter misc_structure 2292..2881 /label=IRES promoter 5191..5458 /label=SV40\Promoter BASE COUNT 1802 a 2104 c 2010 g 1864 t ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga 2281 tccggaattc cgcccccccc cccccccccc ctctccctcc ccccccccta acgttactgg 2341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 2401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 2461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 2521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 2581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 2641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 2701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 2761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 2821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 2881 agcttgccac aacccgtacc aaagatggat agatccggaa agcctgaact caccgcgacg 2941 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 3001 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 3061 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 3121 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 3181 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 3241 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag 3301 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 3361 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 3421 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc 3481 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc 3541 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc 3601 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc 3661 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt 3721 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg 3781 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc 3841 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga 3901 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag 3961 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag 4021 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct 4081 gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg 4141 gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc 4201 cgcccgtgcc tgccgattgg gtgcagatct ctatcgaggg ctgcagcgct gcagaggccg 4261 agtgcagaac tgctccaaag ggacctcaag gctttccgag ggacactagg ctgactccat 4321 cgagccagtg tagagataag cttatcgatt agtccaattt gttaaagaca ggatatcagt 4381 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc 4441 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac 4501 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata 4561 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga 4621 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 4681 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 4741 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 4801 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 4861 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 4921 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 4981 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5101 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5161 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 5221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 5281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 5341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 5401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 5461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt 5521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 5581 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 5641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 6001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6841 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 6901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 6961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7141 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 7201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 7501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 7561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 7621 caagaattag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt 7681 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa 7741 tcaattacgg ggtcattagt tcatagccca tatatggagt
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