出品公司: | Clontech |
---|---|
载体名称: | pLNCX2 |
质粒类型: | 哺乳动物细胞逆病毒基因表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6132bp |
5' 测序引物及序列: | pLNCX-F: AGCTGGTTTAGTGAACCGTCAGATC |
3' 测序引物及序列: | pLNCX-R: ACCTACAGGTGGGGTCTTTCATTCCC |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | -- |
备注: | 可以用来构建稳表达细胞系。 |
产品目录号: | 631503/ 631508/ 631511 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLNCX2 6132 bp DNA circular 4-DEC-2009 COMMENT Created by Clontech Laboratories Inc. COMMENT Cat. No. 631503,631511,631508,631459 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers LTR 1..589 /label=5'\MMSV\LTR LTR 3034..3627 /label=3'\MMLV\LTR misc_signal 659..1468 /label=Extended\packaging\signal CDS 1512..2306 /label=Neo(R) promoter 2373..2905 /label=CMV\IE\Promoter misc_feature 2925..2995 /label=MCS rep_origin 4163..4163 /label=pUC\origin CDS complement(4926..5783) /label=Amp(R) BASE COUNT 1419 a 1665 c 1575 g 1473 t ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatgag 1501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 1561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 1621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 1681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 1741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 1801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 1861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 1921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 1981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 2041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 2101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 2161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 2221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 2281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaatagt aatcaattac 2341 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 2401 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 2461 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 2521 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 2581 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 2641 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 2701 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 2761 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 2821 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 2881 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag 2941 cttgtttggc cgaggcggcc gcttgtcgac aggccttaat ggcctaacat cgataaaata 3001 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg 3061 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga 3121 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc 3181 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg 3241 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg 3301 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg 3361 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg 3421 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg 3481 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct 3541 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg 3601 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc 3661 cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga 3721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 3781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 3841 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 3901 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 3961 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 4081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 4141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 4201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 4261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 4321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 4381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 4501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 4561 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 4621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 5041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 5101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 5161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 5221 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 5281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 5341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 5401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 5461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 5521 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 5581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 5641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5881 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 5941 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcatacca 6001 gatcaccgaa aactgtcctc caaatgtgtc cccctcacac tcccaaattc gcgggcttct 6061 gcctcttaga ccactctacc ctattcccca cactcaccgg agccaaagcc gcggcccttc 6121 cgtttctttg ct
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