pHT43-His枯草芽孢杆菌表达载体

基本信息
启动子: Lac
复制子: ori
质粒分类: 广宿主系列，枯草杆菌载体
质粒大小: 8101bp
原核抗性: Amp
筛选标记: Chl
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 枯草芽孢杆菌
诱导方式: IPTG诱导
5'测序引物: M13F:TGTAAAACGACGGCCAGT
3'测序引物: 根据序列设计引物
质粒简介
   PHT43-His is Bacillus subtilis, the size of 8101bp, which is transformed from the pHT43 vector, screening marks for chloramphenicol.

质粒图谱

质粒序列
LOCUS       Exported                8101 bp ds-DNA     circular SYN 12-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHT43-His
SOURCE      synthetic DNA construct
 ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8101)
 AUTHORS   .
 TITLE     Direct Submission
 JOURNAL   Exported Friday, September 9, 2016 from SnapGene Viewer 3.1.4
           http://www.biovector.net
FEATURES             Location/Qualifiers
    source          1..8101
                    /organism="synthetic DNA construct"
                    /mol_type="other DNA"
    primer_bind     1238..1254
                    /note="M13 fwd"
                    /note="common sequencing primer, one of multiple similar
                    variants"
    CDS             complement(1297..2379)
                    /codon_start=1
                    /gene="lacI"
                    /product="lac repressor"
                    /note="lacI"
                    /note="The lac repressor binds to the lac operator to
                    inhibit transcription in E. coli. This inhibition can be
                    relieved by adding lactose or
                    isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                    /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                    NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                    EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                    EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                    MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                    YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                    ALADSLMQLARQVSRLESGQ"
    protein_bind    2763..2787
                    /bound_moiety="lac repressor encoded by lacI"
                    /note="lac operator"
                    /note="The lac repressor binds to the lac operator to
                    inhibit transcription in E. coli. This inhibition can be
                    relieved by adding lactose or
                    isopropyl-beta-D-thiogalactopyranoside (IPTG)."
    misc_feature    2809..2901
                    /note="SP amyQ"
    CDS             2959..2976
                    /codon_start=1
                    /product="6xHis affinity tag"
                    /note="6xHis"
                    /translation="HHHHHH"
    promoter        6178..6282
                    /gene="bla"
                    /note="AmpR promoter"
    CDS             6283..7143
                    /codon_start=1
                    /gene="bla"
                    /product="beta-lactamase"
                    /note="AmpR"
                    /note="confers resistance to ampicillin, carbenicillin, and
                    related antibiotics"
                    /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                    ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                    PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                    EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                    LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                    LIKHW"
    rep_origin      7314..7902
                    /direction=RIGHT
                    /note="ori"
                    /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                    replication"
ORIGIN
       1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
      61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
     121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
     181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
     241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
     301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
     361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
     421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
     481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
     541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
     601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
     661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
     721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
     781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
     841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
     901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa
     961 aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct
    1021 tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc gccagtcact
    1081 atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    1141 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    1201 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
    1261 gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    1381 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
    1441 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
    1501 aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt cggtatcgtc
    1561 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
    1621 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
    1681 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
    1741 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
    1801 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
    1861 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
    1921 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
    1981 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
    2041 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
    2101 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
    2161 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
    2221 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
    2281 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
    2341 ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct ccctccgttt
    2401 gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta cagtgttatg
    2461 gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac tcaaacatca
    2521 aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg caaccgtttt
    2581 ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag ctattgtaac
    2641 ataatcggta cgggggtgaa aaagctaacg gaaaagggag cggaaaagaa tgatgtaagc
    2701 gtgaaaaatt ttttatctta tcacttgaaa ttggaaggga gattctttat tataagaatt
    2761 gtggaattgt gagcggataa caattcccaa ttaaaggagg aaggatcaat gattcaaaaa
    2821 cgaaagcgga cagtttcgtt cagacttgtg cttatgtgca cgctgttatt tgtcagtttg
    2881 ccgattacaa aaacatcagc cgtaggatcc atgcggccgc taactagtgt caccggtatc
    2941 tagagtcgac gtcccgggca tcaccatcat catcaccagc ccgcctaatg agcgggcttt
    3001 tttcacgtca cgcgtccatg gagatctttg tctgcaactg aaaagtttat accttacctg
    3061 gaacaaatgg ttgaaacata cgaggctaat atcggcttat taggaatagt ccctgtacta
    3121 ataaaatcag gtggatcagt tgatcagtat attttggacg aagctcggaa agaatttgga
    3181 gatgacttgc ttaattccac aattaaatta agggaaagaa taaagcgatt tgatgttcaa
    3241 ggaatcacgg aagaagatac tcatgataaa gaagctctaa aactattcaa taaccttaca
    3301 atggaattga tcgaaagggt ggaaggttaa tggtacgaaa attaggggat ctacctagaa
    3361 agccacaagg cgataggtca agcttaaaga acccttacat ggatcttaca gattctgaaa
    3421 gtaaagaaac aacagaggtt aaacaaacag aaccaaaaag aaaaaaagca ttgttgaaaa
    3481 caatgaaagt tgatgtttca atccataata agattaaatc gctgcacgaa attctggcag
    3541 catccgaagg gaattcatat tacttagagg atactattga gagagctatt gataagatgg
    3601 ttgagacatt acctgagagc caaaaaactt tttatgaata tgaattaaaa aaaagaacca
    3661 acaaaggctg agacagactc caaacgagtc tgttttttta aaaaaaatat taggagcatt
    3721 gaatatatat tagagaatta agaaagacat gggaataaaa atattttaaa tccagtaaaa
    3781 atatgataag attatttcag aatatgaaga actctgtttg tttttgatga aaaaacaaac
    3841 aaaaaaaatc cacctaacgg aatctcaatt taactaacag cggccaaact gagaagttaa
    3901 atttgagaag gggaaaaggc ggatttatac ttgtatttaa ctatctccat tttaacattt
    3961 tattaaaccc catacaagtg aaaatcctct tttacactgt tcctttaggt gatcgcggag
    4021 ggacattatg agtgaagtaa acctaaaagg aaatacagat gaattagtgt attatcgaca
    4081 gcaaaccact ggaaataaaa tcgccaggaa gagaatcaaa aaagggaaag aagaagttta
    4141 ttatgttgct gaaacggaag agaagatatg gacagaagag caaataaaaa acttttcttt
    4201 agacaaattt ggtacgcata taccttacat agaaggtcat tatacaatct taaataatta
    4261 cttctttgat ttttggggct attttttagg tgctgaagga attgcgctct atgctcacct
    4321 aactcgttat gcatacggca gcaaagactt ttgctttcct agtctacaaa caatcgctaa
    4381 aaaaatggac aagactcctg ttacagttag aggctacttg aaactgcttg aaaggtacgg
    4441 ttttatttgg aaggtaaacg tccgtaataa aaccaaggat aacacagagg aatccccgat
    4501 ttttaagatt agacgtaagg ttcctttgct ttcagaagaa cttttaaatg gaaaccctaa
    4561 tattgaaatt ccagatgacg aggaagcaca tgtaaagaag gctttaaaaa aggaaaaaga
    4621 gggtcttcca aaggttttga aaaaagagca cgatgaattt gttaaaaaaa tgatggatga
    4681 gtcagaaaca attaatattc cagaggcctt acaatatgac acaatgtatg aagatatact
    4741 cagtaaagga gaaattcgaa aagaaatcaa aaaacaaata cctaatccta caacatcttt
    4801 tgagagtata tcaatgacaa ctgaagagga aaaagtcgac agtactttaa aaagcgaaat
    4861 gcaaaatcgt gtctctaagc cttcttttga tacctggttt aaaaacacta agatcaaaat
    4921 tgaaaataaa aattgtttat tacttgtacc gagtgaattt gcatttgaat ggattaagaa
    4981 aagatattta gaaacaatta aaacagtcct tgaagaagct ggatatgttt tcgaaaaaat
    5041 cgaactaaga aaagtgcaat aaactgctga agtatttcag cagttttttt tatttagaaa
    5101 tagtgaaaaa aatataatca gggaggtatc aatatttaat gagtactgat ttaaatttat
    5161 ttagactgga attaataatt aacacgtaga ctaattaaaa tttaatgagg gataaagagg
    5221 atacaaaaat attaatttca atccctatta aattttaaca agggggggat taaaatttaa
    5281 ttagaggttt atccacaaga aaagacccta ataaaatttt tactagggtt ataacactga
    5341 ttaatttctt aatgggggag ggattaaaat ttaatgacaa agaaaacaat cttttaagaa
    5401 aagcttttaa aagataataa taaaaagagc tttgcgatta agcaaaactc tttacttttt
    5461 cattgacatt atcaaattca tcgatttcaa attgttgttg tatcataaag ttaattctgt
    5521 tttgcacaac cttttcagga atataaaaca catctgaggc ttgttttata aactcagggt
    5581 cgctaaagtc aatgtaacgt agcatatgat atggtatagc ttccacccaa gttagccttt
    5641 ctgcttcttc tgaatgtttt tcatatactt ccatgggtat ctctaaatga ttttcctcat
    5701 gtagcaaggt atgagcaaaa agtttatgga attgatagtt cctctctttt tcttcaactt
    5761 ttttatctaa aacaaacact ttaacatctg agtcaatgta agcataagat gtttttccag
    5821 tcataatttc aatcccaaat cttttagaca gaaattctgg acgtaaatct tttggtgaaa
    5881 gaattttttt atgtagcaat atatccgata cagcaccttc taaaagcgtt ggtgaatagg
    5941 gcattttacc tatctcctct cattttgtgg aataaaaata gtcatattcg tccatctacc
    6001 tatcctatta tcgaacagtt gaacttttta atcaaggatc agtccttttt ttcattattc
    6061 ttaaactgtg ctcttaactt taacaactcg atttgttttt ccagatctcg agggtaacta
    6121 gcctcgccga tcccgcaaga ggcccggcag tcaggtggca cttttcgggg aaatgtgcgc
    6181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    6241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    6301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    6361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    6421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    6481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    6541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    6601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    6661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    6721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    6781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    6841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    6901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    6961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    7021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    7081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    7141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    7201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    7261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    7321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    7381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    7441 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    7501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    7561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    7621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    7681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    7741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    7801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    7861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    7921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    7981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    8041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcatg
    8101 c
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