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pX552质粒载体CRISPR/Cas9系统图谱序列抗性说明书

  • 价　　格：¥1920
  • 货　　号：pX552 CRISPR/Cas9
  • 产　　地：北京
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pX552质粒载体CRISPR/Cas9系统

PX552
(BioVector-060958)

Print
PX5525303 bp1327885443530348614419397835363094265222101768pBR322_originORF frame 2AmpicillinAmpR_promoterpGEX_3_primerf1_originPmlI (2546)hGH_PA_terminatorClaI (2020)HindIII (2013)EcoRV (2009)EcoRI (2001)BclI (1837)EGFP_C_primerY66 (EGFP)EGFP_N_primerEGFPORF frame 3NcoI (1015)SalI (1005)BamHI (993)KpnI (991)SacII (710)ApaI (522)SacI (417)NdeI (334)LKO1_5_primerhU6_promoter

BACKBONE

  • Vector backbone
    pAAV

  • Backbone size w/o insert (bp)2905

  • Vector type
    Mammalian Expression, Mouse Targeting, AAV, CRISPR

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

SEQUENCE INFORMATION

GENE/INSERT 1

  • Gene/Insert name
    U6_(SpaI)_sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    351

  • PromoterU6

CLONING INFORMATION FOR GENE/INSERT 1

  • Cloning methodGibson Cloning

  • 5′ sequencing primercgcgtgagggcctatttcc

  • 3′ sequencing primergcaagtgggttttaggaccag

    • (Common Sequencing Primers)

GENE/INSERT 2

  • Gene/Insert name
    Syn_EGFP-KASH
  • Species
    Synthetic
  • Insert Size (bp)
    349

  • PromoterSynapsin

CLONING INFORMATION FOR GENE/INSERT 2

  • Cloning methodGibson Cloning

  • 5′ sequencing primerctgcgtatgagtgcaagtgggtt

  • 3′ sequencing primerccgttggtgtaccgcagcatg

    • (Common Sequencing Primers)

RESOURCE INFORMATION


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