首页 » pX552质粒载体CRISPR/Cas9系统图谱序列抗性说明书

pX552质粒载体CRISPR/Cas9系统图谱序列抗性说明书

  • 价  格:¥1920
  • 货  号:pX552 CRISPR/Cas9
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

pX552质粒载体CRISPR/Cas9系统

PX552
(BioVector-060958)

PX5525303 bp1327885443530348614419397835363094265222101768pBR322_originORF frame 2AmpicillinAmpR_promoterpGEX_3_primerf1_originPmlI (2546)hGH_PA_terminatorClaI (2020)HindIII (2013)EcoRV (2009)EcoRI (2001)BclI (1837)EGFP_C_primerY66 (EGFP)EGFP_N_primerEGFPORF frame 3NcoI (1015)SalI (1005)BamHI (993)KpnI (991)SacII (710)ApaI (522)SacI (417)NdeI (334)LKO1_5_primerhU6_promoter

BACKBONE

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp)2905

  • Vector type
    Mammalian Expression, Mouse Targeting, AAV, CRISPR

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

SEQUENCE INFORMATION

GENE/INSERT 1

  • Gene/Insert name
    U6_(SpaI)_sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    351
  • PromoterU6

CLONING INFORMATION FOR GENE/INSERT 1

  • Cloning methodGibson Cloning

  • 5′ sequencing primercgcgtgagggcctatttcc

  • 3′ sequencing primergcaagtgggttttaggaccag

  • (Common Sequencing Primers)

GENE/INSERT 2

  • Gene/Insert name
    Syn_EGFP-KASH
  • Species
    Synthetic
  • Insert Size (bp)
    349
  • PromoterSynapsin

CLONING INFORMATION FOR GENE/INSERT 2

  • Cloning methodGibson Cloning

  • 5′ sequencing primerctgcgtatgagtgcaagtgggtt

  • 3′ sequencing primerccgttggtgtaccgcagcatg

  • (Common Sequencing Primers)

RESOURCE INFORMATION


您正在向 biovector.net  发送关于产品 pX552质粒载体CRISPR/Cas9系统图谱序列抗性说明书 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。