pX551质粒载体CRISPR/Cas9系统图谱序列抗性说明书

价　　格：¥1920
货　　号：pX551 CRISPR/Cas9
产　　地：北京

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pX551质粒载体CRISPR/Cas9系统

PX551
(BioVector-060957)

PURPOSE
pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
DEPOSITING LAB
Feng Zhang
PUBLICATION
Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.

BACKBONE

Vector backbone
pAAV
(Search Vector Database)
Backbone size w/o insert (bp)2905
Vector type
Mammalian Expression, AAV, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Stbl3
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences
Full (1)
Addgene Sequences
Partial (3)

GENE/INSERT

Gene/Insert name
SpCas9
Alt name
S. pyogenes Cas9 nuclease
Alt name
Cas9
Species
Synthetic
Insert Size (bp)
4200
PromoterpMecp2
Tag / Fusion Protein
- HA (N terminal on insert)

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerAATGGGGTCCGCCTCTTTTCC
3′ sequencing primercttagctggcctccacctttctcttc

(Common Sequencing Primers)

RESOURCE INFORMATION

Supplemental documents
- PX551
Terms and Licenses
- UBMTA

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:
PX551 was a gift from Feng Zhang (Addgene plasmid # 60957)
For your References section:
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.10.1038/nbt.3055 PubMed 25326897