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pX551质粒载体CRISPR/Cas9系统图谱序列抗性说明书

  • 价  格:¥1920
  • 货  号:pX551 CRISPR/Cas9
  • 产  地:北京
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pX551质粒载体CRISPR/Cas9系统


PX551
(BioVector-060957)

PX5517400 bp18511234618740067836167555049344317370130842467pBR322_originSacII (6442)AseI (6332)FspI (6284)ORF frame 2AmpicillinAmpR_promoterpGEX_3_primerf1_originNarI (4805)NotI (4656)EcoRI (4601)PmlI (3250)SacI (2700)EcoRV (2314)ApaI (1820)NLSSV40 NLSHAORF frame 2AgeI (389)ORF frame 2HindIII (154)XbaI (148)

BACKBONE

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp)2905

  • Vector type
    Mammalian Expression, AAV, CRISPR

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

SEQUENCE INFORMATION

GENE/INSERT

  • Gene/Insert name
    SpCas9
  • Alt name
    S. pyogenes Cas9 nuclease
  • Alt name
    Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4200
  • PromoterpMecp2

  • Tag / Fusion Protein

    • HA (N terminal on insert)


CLONING INFORMATION

  • Cloning methodRestriction Enzyme

  • 5′ cloning siteAgeI (not destroyed)

  • 3′ cloning siteEcoRI (not destroyed)

  • 5′ sequencing primerAATGGGGTCCGCCTCTTTTCC

  • 3′ sequencing primercttagctggcctccacctttctcttc

  • (Common Sequencing Primers)

RESOURCE INFORMATION

  • Supplemental documents
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX551 was a gift from Feng Zhang (Addgene plasmid # 60957)
  • For your References section:

    In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.10.1038/nbt.3055 PubMed 25326897


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