Thialkalivibrio versutus DSM No.: 13738 BioVector NTCC质粒载体菌种细胞基因保藏中心

Thialkalivibrio versutus DSM No.: 13738 BioVector NTCC质粒载体菌种细胞基因保藏中心 Name: Thioalkalivibrio versutus Sorokin et al. 2001 Synonym(s): Thialkalivibrio versutus corrig. Sorokin et al. 2001 DSM No.: 13738, Type strain Strain designation: AL2 Other collection no. or WDCM no.: CBS 100464 Isolated from: soda lake Geographic origin: Russia History: <- D. Y. Sorokin; AL2 Genbank accession numbers: 16S rRNA gene: AF126546 16S rRNA gene: FR749906 Cultivation conditions: Medium 925 , 30°C Summary and additional information: <- D. Y. Sorokin; AL2. (Thialkalivibrio versutus). Soda lake; Russia.Type strain. Taxonomy/description (8049). Sequence accession no. 16S rRNA gene: AF126546 (this sequence may contain a number of errors, D.Y. Sorokin pers. comm.), 16S rRNA gene: FR749906. (Medium 925, 30°C). For detailed information: BacDive - The Bacterial Diversity Metadatabase Literature: Sorokin, D. Y., Lysenko, A. M., Mityushina, L. L., Tourova, T. P., Jones, B. E., Rainey, F. A., Robertson, L. A., Kuenen, G. J. (2001). Thioalkalimicrobium aerophilum gen. nov., sp. nov. and Thioalkalimicrobium sibericum sp. nov., and Thioalkalivibrio versutus gen. nov., sp. nov.,Thioalkalivibrio nitratis sp. nov. and Thioalkalivibrio denitrificans sp. nov., novel obligately alkaliphilic and obligately chemolithoautotrophic sulfur-oxidizing bacteria from soda lakes.Int.J.Syst.Evol.Microbiol. 51 : 565-580 . http://www.ncbi.nlm.nih.gov/pubmed/11321103 Risk group: 1 925. ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM Mineral base: Na2CO3 20.0 g NaHCO3 10.0 g NaCl 5.0 g K2HPO4 1.0 g Distilled water 1000.0 ml Sterilize at 110C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. Trace element solution: EDTA 5.0 mg FeSO4 x 7 H2O 2.0 mg ZnSO4 x 7 H2O 100.0 mg MnCl2 x 4 H2O 30.0 mg CoCl2 x 6 H2O 200.0 mg NiCl2 x 6 H2O 20.0 mg Na2MoO4 x 2 H2O 30.0 mg CuCl2 x 2 H2O 10.0 mg H3BO3 300.0 mg Distilled water 1000.0 ml Final pH should be 3, add HCl if needed. Sterilization - 120C 20 min. After sterilization add: Trace elements solution 2 ml/l MgCl2 x 6 H2O (200.0 g/l) 1 ml/l (a white colloid will form which will rapidly dissolve after mixing) Prepare sterile stock solutions of the following Sodium thiosulfate pentahydrate (2 M - 496 g/l) KSCN solution (2 M - 194 g/l) NH4Cl - (1 M - 53.5 g/l) KNO3 - (1 M - 101 g/l) Growth: DSM 13531 = ARh1 (KSCN grown): Growth with thiocyanate : add KSCN up to 15 mM, incubation - 30C in conical flasks 1/10 liquid to air ratio, statically; growth is slow, about 1 week DSM 13541 = ARh2 (thiosulphate grown): Growth with thiosulfate: add 40 mM thiosulfate and 5 mM NH4Cl, incubate in closed flasks, 1/10 liquid to air on shaker 200 rpm; heavy sulfur formation is usual, it is necessary to wait until all the sulfur is consumed (about 1 week). DSM 13532 = ARh2 (KSCN grown): Growth with 15 mM KSCN: same conditions as for DSM 13531 = ARh1. DSM 13542 = ARh1 (thiosulphate grown) Growth with thiosulfate (40 mM) and nitrate (10 mM); in conical flasks 1/5 liquid/air ratio on shaker 200 rpm. Growth takes 3-4 days, some sulfur can be formed during initial growth phase. DSM 13533 = ALRh strains ALRh: grows with 40 mM thiosulfate and 5 mM KSCN in conical flasks 1/5 liquid to air ratio at 200 rpm. Thioalkalivibrio versutus AL 2 = DSM 13738, Tv.nitratus ALJ 12 = DSM 13741, and Tv.denitrificans ALJD = DSM 13742 Use 40 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM Thioalkalimicrobium aerophilum AL 3 = DSM 13739 and Tm.sibericum AL 7 = DSM 13740 Use 80 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM Thioalkalispira microaerophila, ALEN 1 = DSM 14786. The strain is grown under nitrogen with 1% oxygen in the gas phase. The strain grows slowly with a potential lag phase of several days. Use 30 mM thiosulphate and up to a maximum of 5 mM NH4Cl Thioalkalivibrio nitratireducens ALEN 2 = DSM 14787 Use 30 - 40 mM thiosulphate and 5-10 mM KNO3 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net