HL-60 cells BioVector NTCC质粒载体菌种细胞基因保藏中心

HL-60 cells BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line: HL-60 DSMZ no.: ACC 3Species: human (Homo sapiens)Cell type: acute myeloid leukemia Origin: established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described to be responsive to DMSO, phorbol ester TPA and other reagents (e.g. expression of TNF alpha) and to carry amplified MYC gene; present cells are apparently tetraploid derivates of hypodiploid original where MYC was amplified in dmin (instead of hsr) Reference(s): 14548, 14437, 14438, 14443Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:round, single cells in suspension; image; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS Subculture: maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:5 every 1-2 days; seed out at about 1 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: about 40 hours Harvest: maximal density of 1.5-2.0 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of MDH, NP Cytogenetics: human flat-moded hypotetraploid karyotype with hypodiploid sideline and 1.5% polyploidy - 82-88<4n>XX, -X, -X, -8, -8, -16, -17, -17, +18, +22, +2mar, ins(1;8)(p?31;q24hsr)x2, der(5)t(5;17)(q11;q11)x2, add(6)(q27)x2, der(9)del(9)(p13)t(9;14)(q?22;q?22)x2, der(14)t(9;14)(q?22;q?22)x2, der(16)t(16;17)(q22;q22)x1-2, add(18)(q21) - sideline with: -2, -5, -15, del(11)(q23.1q23.2) - c-myc amplicons present in der(1) and in both markers Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 - , HTLV-I/II -, MLV -, SMRV - [Supplier来源] http://www.biovector.net