L-929 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心

L-929 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心 L-929 Cells Cat No.:NTCC595657/ACC2 Cell Line Name: L929 Keywords: Mouse C3H/An connective tissue Cell Line Description: The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse by WR Earle in 1940. NCTC clone 929 Clone of strain L (also known as L929) was subsequently derived in March 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain. Cells are APRT+ and HPRT+. Species: Mouse Tissue: Adipose Morphology: Fibroblast Growth Mode: Adherent Subculture Routine: Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Karyotype: Not specified Originator: No Country: UK References: Earle WR, et al. 1943 Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212. Additional Bibliography: Sanford KK, et al. 1948 The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246.EARLE WR, SANFORD KK, EVANS VJ, WALTZ HK, SHANNON JE Jr.1951 The influence of inoculum size on proliferation in tissue cultures.J Natl Cancer Inst. 12(1):133-53.PMID:14874126. SANFORD KK, HOBBS GL, EARLE WR.1956 The tumor-producing capacity of strain L mouse cells after 10 years in vitro.Cancer Res. 16(2):162-6.PMID:13293658. [Supplier来源] http://www.biovector.net