pGEM5Z(+)-EGFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pGEM5Z(+)-EGFP BioVector NTCC质粒载体菌种细胞基因保藏中心 Biovector565206 pGEM5Z(+)-EGFP pGEM5Z(+)-EGFP,AmpR.1vial,in E.coli. Storage:4℃ EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. • Vector backbone pGEM5Z(+) • Backbone manufacturer Promega • Backbone size w/o insert (bp)3001 • Total vector size (bp)4604 • Vector type Mammalian Expression, Bacterial Expression • Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) DH5alpha • Copy number High Copy GENE/INSERT • Gene/Insert name EGFP • Species Aequorea victoria • Insert Size (bp) 1632 • Mutation Restriction enzyme recognition sites added either side of the EGFP start codon (EcoRV upstream, NruI downstream) • PromoterCMV CLONING INFORMATION • Cloning methodRestriction Enzyme • 5′ cloning siteNdeI (unknown if destroyed) • 3′ cloning siteEcoRV (unknown if destroyed) • 5′ sequencing primerSP6 • 3′ sequencing primerT7 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net