p189 (BioVector-065207) BioVector NTCC质粒载体菌种细胞基因保藏中心

p189 (BioVector-065207) BioVector NTCC质粒载体菌种细胞基因保藏中心 Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). • Vector backbone pGEM5Z(+) • Backbone manufacturer Promega • Backbone size w/o insert (bp)3001 • Total vector size (bp)4604 • Vector type Mammalian Expression, Bacterial Expression • Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) DH5alpha • Copy number High Copy GENE/INSERT • Gene/Insert name EGFP • Species Aequorea victoria • Insert Size (bp) 1632 • Mutation Premature stop codon added to pGEM5Z(+)-EGFP (TGG(58)-->TAG) • PromoterCMV CLONING INFORMATION • Cloning methodRestriction Enzyme • 5′ cloning siteNdeI (unknown if destroyed) • 3′ cloning siteEcoRV (unknown if destroyed) • 5′ sequencing primerSP6 • 3′ sequencing primerT7 . BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net