pBAD-LacI (BioVector-046394) BioVector NTCC质粒载体菌种细胞基因保藏中心

pBAD-LacI (BioVector-046394) BioVector NTCC质粒载体菌种细胞基因保藏中心 Overproduction of LacI. LacI expression was induced with 0.2% (w/v) L-arabinose and cells incubated for 4 h at 20 °C. Vector backbone pBADmyc-hisB (Search Vector Database) Backbone manufacturer Invitrogen Backbone size w/o insert (bp) 4092 Total vector size (bp) 5080 Vector type Bacterial Expression GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) Top10 Copy number Unknown GENE/INSERT Gene/Insert name LacI Species Escherichia coli Insert Size (bp) 1110 Mutation silent T to C mutation at position 19 Promoter arabinose promoter Tag / Fusion Protein 6xHIS tag (C terminal on insert) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site NcoI (unknown if destroyed) 3′ cloning site SalI (unknown if destroyed) 5′ sequencing primer pBADseqF (5' AGCGGATCCTACCTGACG) 3′ sequencing primer pBADseqR (5' CTGAAAATCTTCTCTCATCCG) [Supplier来源] http://www.biovector.net