pKA-207I10 (BioVector-079845) BioVector NTCC质粒载体菌种细胞基因保藏中心

pKA-207I10 (BioVector-079845) BioVector NTCC质粒载体菌种细胞基因保藏中心 Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter. Vector backbone pIRES-mCherry (Search Vector Database) Backbone manufacturer Clontech Backbone size w/o insert (bp) 4000 Total vector size (bp) 10100 Modifications to backbone IRES strength was reduced ~ 3-fold Vector type Mammalian Expression Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA Bacterial Resistance(s) Kanamycin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR Species Rhodopseudomonas palustris Insert Size (bp) 6200 Mutation RpPpsR2 cysteine 439 changed to serine GenBank IDKX063612 KX063613 Promoter CMV CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site NheI (not destroyed) 3′ cloning site NotI (not destroyed) 5′ sequencing primer ggtaggcgtgtacggtgggag. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net