HT-2 clone A5E cell line细胞株/完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1841)

价　　格：¥89735
货　　号：HT-2 clone A5E cell line细胞株/完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1841)
产　　地：北京

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HT2 Clone A5E小鼠辅助T细胞细胞 BioVector NTCC保藏中心
HT2 Clone A5E Mouse helper T cell cell line

HT-2 clone A5E cell line

Cat No.: BioVector-A1841

Product category

Animal cells

Organism

Mus musculus, mouse

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Cell type

lymphocyte

Morphology

lymphoblast

Tissue

Spleen

Ap plications

3D cell culture

Immunology

Specific applications

The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay.

The cells are commonly used for characterization of lymphokines and cytokines.

Growth properties

Suspension

Derivation

The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of C57BL spleen cells activated by sheep

erythrocytes in the presence of IL-2.

This cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at BioVector and by the NIST Mouse STR

Consortium using mouse STR technology has shown that the STR profile has more alleles in common with a C57BL mouse strain rather

than BALB/c.

Strain

C57BL

An tigen expression

Thy-1Biovector NTCC, Inc

No.19,Xizhimen,Beijing,China

http://www.Biovector.net

Tel:010-53513060. 400-800-2947

Expression m arkers

Interleukin 2 (IL-2)

Com m ents

The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.

In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).

Com plete m edium

The complete medium for culturing BioVector-A1841 is composed of:

399.5 mL RPMI-1640

50 mL FBS

0.5 mL 2-Mercaptoethanol

50 mL Rat T-STIM with Con A

Tem perature

37°C

【Organism物种来源】Animal
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doubling Time倍增殖时间】
【References参考文献】

【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net