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Special
Characteristics:
The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either β-gal
or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran
in the infection medium. The ß-gal and luciferase genes are also induced by HIV-2 infection.
Cell Type:
TZM-bl, previously designated JC53-bl (clone 13) is a HeLa cell line. The parental cell line
(JC.53) stably expresses large amounts of CD4 and CCR5. The TZM-bl cell line was generated
from JC.53 cells by introducing separate integrated copies of the luciferase and
ß
-galactosidase genes under control of the HIV-1 promoter. The TZM-bl cell line is highly
sensitive to infection with diverse isolates of HIV-1.
Freeze Medium:
50% FBS, 40% DMEM, 10% DMSO.
Growth
Characteristics:
Adherent cell line. No special requirements for thawing and reestablishing the culture. Cells
grow in single cell layer. Antibiotic selection is not required to maintain stable expression of the
receptors and reporter genes.
Propagation
Medium:
DMEM (90%), 10% FBS, 100 units of Penicillin and 0.1 mg/ml of Streptomycin.
Sterility:
Negative for bacteria, mycoplasma, and fungi.
References:
Platt EJ, Bilska M, Kozak SL, Kabat D, Montefiori DC. Evidence that ecotropic murine leukemia
virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays
with human immunodeficiency virus type 1. J Virol.2009;83(16):8289-92
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